CAJ | 학술논문

[目的]探讨红霉素、环丙沙星、左氧氟沙星、莫西沙星4种抗菌药物对细胞内嗜肺军团菌的抗菌活性.[方法]分别采用E-test法和有限稀释法测定上述4种药物对嗜肺军团菌ATCC 33152的细胞外抗菌活性,再用噻唑蓝比色法(MTT法)测定上述药物对嗜肺军团菌ATCC 33152的细胞内抗菌活性.[结果]细胞外抗菌活性E-test法:红霉素0.047 μg/mL、环丙沙星.38μg/mL、左氧氟沙星0.125μg/mL、莫西沙星0.125μg/mL;有限稀释法:红霉素0.125μg/mL、环丙沙星0.03μg/mL、左氧氟沙星0.016 μg/mL、莫西沙星0.016 μg/mL.4种药物的细胞内抗菌活性分别为:红霉素0.25 μg/mL、环丙沙星0.016 μg/mL、左氧氟沙星0.016 μg/mL、莫西沙星0.004 μg/mL.[结论]氟喹诺酮类药物在U937细胞模型中对嗜肺军团菌ATCC 33152具有很强的细胞内抗菌活性.其中莫西沙星的细胞内抗菌活性最强.MTT法是一种行之有效的用于检测药物对细胞内嗜肺军团菌抗菌活性的方法,可同时检测并对比各种药物样本的细胞内抗菌活性.
[목적]탐토홍매소、배병사성、좌양불사성、막서사성4충항균약물대세포내기폐군단균적항균활성.[방법]분별채용E-test법화유한희석법측정상술4충약물대기폐군단균ATCC 33152적세포외항균활성,재용새서람비색법(MTT법)측정상술약물대기폐군단균ATCC 33152적세포내항균활성.[결과]세포외항균활성E-test법:홍매소0.047 μg/mL、배병사성.38μg/mL、좌양불사성0.125μg/mL、막서사성0.125μg/mL;유한희석법:홍매소0.125μg/mL、배병사성0.03μg/mL、좌양불사성0.016 μg/mL、막서사성0.016 μg/mL.4충약물적세포내항균활성분별위:홍매소0.25 μg/mL、배병사성0.016 μg/mL、좌양불사성0.016 μg/mL、막서사성0.004 μg/mL.[결론]불규낙동류약물재U937세포모형중대기폐군단균ATCC 33152구유흔강적세포내항균활성.기중막서사성적세포내항균활성최강.MTT법시일충행지유효적용우검측약물대세포내기폐군단균항균활성적방법,가동시검측병대비각충약물양본적세포내항균활성.
[Objective]To explore the intracellular antimicrobial activities of erythromycin,ciprofloxacin,levofloxacin,moxifloxacin against Legionella pneumophila.[Methods]The minimum inhibition concentration(MIC)of each antibiotic was evaluated by E-test method and microdilution method respectively.The minimal extracellular concentration inhibiting intracellular multiplication(MIEC)of each antibiotic was evaluated by the MTT colorimetric assay system.[Results]The MIC concentration for each drug by E-test method were:erythromycin,0.047 μg/mL;ciprofloxacin,0.38 μg/mL;levofloxacin,0.125 μg/mL;moxifloxacin,0.125 μg/mL,the MIC concentrations for each drug by microdilution method were:erythromycin,0.125 μg/mL;ciprofloxacin,0.03 μg/mL;levofloxacin,0.016 μg/mL;moxifloxacin,0.016 μg/mL.The MIEC concentration for each drug were:erythromycin,0.25 μg/mL;ciprofloxacin,0.016 μg/mL;levofloxacin,0.016 μg/mL;moxifloxacin,0.004 μg/mL.[Conclusions]Fluoroquinolones have superior activity than erythromycin in U937 cells infected with L.pneumophila.Moxifloxacin is the most potent drug among the four tested antimicrobials.Our results indicated that the MTT assay system allows comparative and quantitative evaluations of the intracellular activities of antibiotics against L,pneumophila and efficient processing of a large number of samples.