CAJ | 학술논문

试验以亚麻、大麻和青麻细胞壁加厚的韧皮纤维为材料,进行细胞骨架制备条件优化研究.即在普通考马斯亮蓝R250染色基础之上,对1%Triton X-100抽提非骨架蛋白时间进行了优化.结果表明,在37℃恒温箱中用1%Triton X-100处理材料30 min,纤维细胞骨架清晰可见.试验为进一步研究细胞骨架系统和纤维素微纤丝如何在细胞壁中沉积等奠定了基础.
시험이아마、대마화청마세포벽가후적인피섬유위재료,진행세포골가제비조건우화연구.즉재보통고마사량람R250염색기출지상,대1%Triton X-100추제비골가단백시간진행료우화.결과표명,재37℃항온상중용1%Triton X-100처리재료30 min,섬유세포골가청석가견.시험위진일보연구세포골가계통화섬유소미섬사여하재세포벽중침적등전정료기출.
The bast fibres with the thicken cell wall of flax, hemp and abutilon were used as test materials to study the cytoskeleton morphology, and to optimize the time of non-matrix proteins by 1% Triton X-100 with coomassie brilliant blue R250 staining. The results showed that cytoskeleton of the fibre cell was clearly visible through 30 min treatment by 1% Triton X-100 at 37℃. This experiment established the foundation for studying cytoskeleton system and how the cellulose microfibril deposied in the cell wall in the future.