CAJ | 학술논문

目的了解河北省婴幼儿腹泻患者轮状病毒的感染特点及基因型别的变迁情况.方法 2007 -2010年在河北卢龙县医院和卢龙县妇幼保健院收集5岁以下腹泻住院病例1643例的粪便标本,采用酶联免疫吸附试验( ELISA)检测轮状病毒抗原,阳性标本用多重反转录-聚合酶链反应(RT-PCR)进行基因分型.结果 1643例标本中801例轮状病毒抗原阳性,阳性率为48.75％.对轮状病毒抗原阳性标本进行G/P分型,2007 -2010年G3型所占比例波动于48.59％～ 69.92％,G1型由2007年的10.59％下降至2010年的5.79％,G9型则由2007年的2.12％上升至2010年的19.74％.P分型以P[8]最为常见,G/P组合以G3P[8]为主.结论轮状病毒是要幼儿腹泻的主要病原,G3P[8]为河北省主要流行株,G9型有上升趋势.
목적 료해하북성영유인복사환자륜상병독적감염특점급기인형별적변천정황.방법 2007 -2010년재하북로룡현의원화로룡현부유보건원수집5세이하복사주원병례1643례적분편표본,채용매련면역흡부시험( ELISA)검측륜상병독항원,양성표본용다중반전록-취합매련반응(RT-PCR)진행기인분형.결과 1643례표본중801례륜상병독항원양성,양성솔위48.75％.대륜상병독항원양성표본진행G/P분형,2007 -2010년G3형소점비례파동우48.59％～ 69.92％,G1형유2007년적10.59％하강지2010년적5.79％,G9형칙유2007년적2.12％상승지2010년적19.74％.P분형이P[8]최위상견,G/P조합이G3P[8]위주.결론 륜상병독시요유인복사적주요병원,G3P[8]위하북성주요류행주,G9형유상승추세.
Objective To investigate the infection characteristics of group A rotavirus in children with diarrhea in Hebei.Methods Stool specimens were collected from 1643 children (less than 5 years old) with diarrhea in Lulong county hospital,Lulong county maternal and duild health hospital from 2007 to 2010.The specimens were detected by ELISA,and the positive samples were genotyped using multiple reverse transeriptase-polymerase chain reaction.Results A total of 801 positive cases were detected among 1643 patients with diarrhea,with the positive rate of 48.75％.All the positive specimens were genotyped for G/P.G3 serotype ranged from 48.59％ to 69.92％,2007-2010.The proportion of G1 dropped from 10.59％ in 2007 to 5.79％ in 2010.However,The proportion of G9 rose from 2.12％ in 2007 to 19.74％ in 2010.P[8 ]was the most common P genotype.G3P[8 ] ranked first in combination of G/P.Conclusion Rotavirus is the main pathogen of infantile diarrhea.G3P[ 8 ] is the major epidemic strains and the G9 serotype has a rising trend.