中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2011年
10期
769-772
,共4页
王燕%刘岩%马剑侠%李宝新%李玉坤
王燕%劉巖%馬劍俠%李寶新%李玉坤
왕연%류암%마검협%리보신%리옥곤
骨质疏松,绝经后%β连环素%LDL受体相关蛋白质类%核心结合因子α1亚基%雌二醇
骨質疏鬆,絕經後%β連環素%LDL受體相關蛋白質類%覈心結閤因子α1亞基%雌二醇
골질소송,절경후%β련배소%LDL수체상관단백질류%핵심결합인자α1아기%자이순
Osteoporosis,postmenopausal%beta-Catenin%LDL-receptor related proteins%Corebinding factor alpha 1 subunit%Estradiol
目的 探讨Wnt/LRPS/β-catenin信号通路在绝经后骨质疏松发病中的作用.方法 选取50只6月龄雌性Wistar大鼠,随机分为对照组(n=24)和去卵巢组(n=26),去卵巢组大鼠行双侧卵巢切除术,对照组打开大鼠腹腔后不作任何处理即缝合.去卵巢后0、4和8周分别测定两组大鼠血雌二醇水平和骨密度,4和8周用逆转录-PCR技术检测骨组织中低密度脂蛋白受体相关蛋白5( LRP5)、β连环蛋白(β-catenin)及骨形成关键基因—runt相关基因2(Runx2)mRNA的表达.结果 去卵巢组大鼠术后4和8周,出现雌二醇水平明显下降,术后4周时为( 92±15) pmol/L,对照组为( 117±29) pmol/L；术后8周时为(95±22) pmol/L,对照组为(114±15) pmol/L；两组分别比较,差异均有统计学意义(P＜0.05).术后4周时去卵巢组骨密度为(0.076±0.016) g/cm2,对照组为(0.098 +0.016) g/cm2;术后8周时去卵巢组为(0.052±0.013) g/cm2,对照组为(0.095±0.028)g/cm2；两组分别比较,差异均有统计学意义(P＜0.01).去卵巢组大鼠术后4周骨组织中LRP5、β-catenin和Runx2 mRNA表达水平明显减少,分别为0.97±0.04、0.58±0.05、0.86±0.03,对照组为1.02±0.06、1.04±0.05、1.07±0.21,两组各指标分别比较,差异均有统计学意义(P＜0.05).结论 Wnt/LRP5/β-catenin信号通路抑制可能是绝经后骨质疏松重要发病机制之一.
目的 探討Wnt/LRPS/β-catenin信號通路在絕經後骨質疏鬆髮病中的作用.方法 選取50隻6月齡雌性Wistar大鼠,隨機分為對照組(n=24)和去卵巢組(n=26),去卵巢組大鼠行雙側卵巢切除術,對照組打開大鼠腹腔後不作任何處理即縫閤.去卵巢後0、4和8週分彆測定兩組大鼠血雌二醇水平和骨密度,4和8週用逆轉錄-PCR技術檢測骨組織中低密度脂蛋白受體相關蛋白5( LRP5)、β連環蛋白(β-catenin)及骨形成關鍵基因—runt相關基因2(Runx2)mRNA的錶達.結果 去卵巢組大鼠術後4和8週,齣現雌二醇水平明顯下降,術後4週時為( 92±15) pmol/L,對照組為( 117±29) pmol/L；術後8週時為(95±22) pmol/L,對照組為(114±15) pmol/L；兩組分彆比較,差異均有統計學意義(P＜0.05).術後4週時去卵巢組骨密度為(0.076±0.016) g/cm2,對照組為(0.098 +0.016) g/cm2;術後8週時去卵巢組為(0.052±0.013) g/cm2,對照組為(0.095±0.028)g/cm2；兩組分彆比較,差異均有統計學意義(P＜0.01).去卵巢組大鼠術後4週骨組織中LRP5、β-catenin和Runx2 mRNA錶達水平明顯減少,分彆為0.97±0.04、0.58±0.05、0.86±0.03,對照組為1.02±0.06、1.04±0.05、1.07±0.21,兩組各指標分彆比較,差異均有統計學意義(P＜0.05).結論 Wnt/LRP5/β-catenin信號通路抑製可能是絕經後骨質疏鬆重要髮病機製之一.
목적 탐토Wnt/LRPS/β-catenin신호통로재절경후골질소송발병중적작용.방법 선취50지6월령자성Wistar대서,수궤분위대조조(n=24)화거란소조(n=26),거란소조대서행쌍측란소절제술,대조조타개대서복강후불작임하처리즉봉합.거란소후0、4화8주분별측정량조대서혈자이순수평화골밀도,4화8주용역전록-PCR기술검측골조직중저밀도지단백수체상관단백5( LRP5)、β련배단백(β-catenin)급골형성관건기인—runt상관기인2(Runx2)mRNA적표체.결과 거란소조대서술후4화8주,출현자이순수평명현하강,술후4주시위( 92±15) pmol/L,대조조위( 117±29) pmol/L；술후8주시위(95±22) pmol/L,대조조위(114±15) pmol/L；량조분별비교,차이균유통계학의의(P＜0.05).술후4주시거란소조골밀도위(0.076±0.016) g/cm2,대조조위(0.098 +0.016) g/cm2;술후8주시거란소조위(0.052±0.013) g/cm2,대조조위(0.095±0.028)g/cm2；량조분별비교,차이균유통계학의의(P＜0.01).거란소조대서술후4주골조직중LRP5、β-catenin화Runx2 mRNA표체수평명현감소,분별위0.97±0.04、0.58±0.05、0.86±0.03,대조조위1.02±0.06、1.04±0.05、1.07±0.21,량조각지표분별비교,차이균유통계학의의(P＜0.05).결론 Wnt/LRP5/β-catenin신호통로억제가능시절경후골질소송중요발병궤제지일.
Objective To investigate the role of the Wnt/LRPS/3-catenin signaling pathway in the pathogenesis of postmenopausal osteoporosis.Methods Fifty female Wistar rats aged 6-month-old,were randomly divided into control group ( NS,n =24 ) and ovariectomized group ( NOVX,n =26),NOVX underwent bilateral ovariectomy.At 0,4 and 8 weeks,all of rats were measured blood estrogen ( E2 ) and bone mineral density (BMD),4 and 8 weeks,low density lipoprotein receptor-related protein 5 (LRP5),3-catenin and Runx2 mRNA in bone were measured respectively by reverse transcription (RT)-PCR.Results In 4 and 8 weeks,compared with NS which had (117±29) and (114+15) pmol/L in E2 level,(0.098 ± 0.016 ) and (0.095 ± 0.028 ) g/cm2 in BMD,NOVX had significantly decreased to ( 92±15 ) and(95±22) pmol/L in E2 level ( P＜0.05 ),( 0.076 ± 0.016) and ( 0.052 ± 0.013 ) g/cm2 in BMD values ( P ＜ 0.01 ).And bone tissue LRP5,β-catenin and Runx2 mRNA expression was 1.02 ± 0.06,1.04 ±0.05,1.07 ±0.21 in NS,NOVX was significantly reduced to 0.97 ± 0.04,0.58 ± 0.05,0.86 ±0.03 (P＜0.05).Conclusion Wnt/LRP5/β-catenin signaling pathway may be important in the pathogenesis of postmenopausal osteoporosis.
