中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2010年
10期
739-743
,共5页
邓洁%钱渊%赵林清%朱汝南%王芳%孙宇%廖斌%黄荣妍%袁艺%曲东%任晓旭
鄧潔%錢淵%趙林清%硃汝南%王芳%孫宇%廖斌%黃榮妍%袁藝%麯東%任曉旭
산길%전연%조림청%주여남%왕방%손우%료빈%황영연%원예%곡동%임효욱
腺病毒,人%腺病毒感染,人%呼吸道感染%儿童
腺病毒,人%腺病毒感染,人%呼吸道感染%兒童
선병독,인%선병독감염,인%호흡도감염%인동
Adenoviruses,human%Adenovirus infections,human%Respiratory tract infections%Child
目的 了解近年北京地区儿科急性呼吸道感染患儿中人腺病毒(ADV)感染状况及ADV的型别.方法 2003年至2008年,连续6年通过病毒分离和(或)间接免疫荧光法,对收集的首都儿科研究所就诊的急性呼吸道感染患儿的17 941份标本进行ADV检测,对其中285份毒株或阳性标本提取DNA,通过3、7、11和21型4对分型引物的多重聚合酶链反应,确定3、7、11和21型ADV,对少数扩增阴性的标本或毒株,再使用2对可扩增所有ADV的通用引物对其DNA进行扩增,将PCR产物直接测序,结果与GenBank上的序列比较,确定其型别.结果 在17 941份呼吸道标本中,经病毒分离和(或)间接免疫荧光法确定为ADV阳性的标本304份,总阳性率为1.69%.对其中285份毒株或阳性标本的PCR分型结果:272例用3、7、11、21型分型引物扩增确定了型别:3型ADV 174例,占61.1%(174/285),7型ADV 92例,占32.3%,11型ADV 6例,占2.1%;有13例经分型引物扩增为阴性,经过通用引物扩增后的PCR产物测序确定为2型ADV 9例,占3.2%(9/285);6型ADV2例,占0.7%,1型和5型ADV各1例,各占0.4%.结论 本组ADV阳性检出率为1.69%.近年来北京地区ADV感染以3、7型为主,其次为2型和11型,1、5和6型较为少见,尚未发现其他型别.
目的 瞭解近年北京地區兒科急性呼吸道感染患兒中人腺病毒(ADV)感染狀況及ADV的型彆.方法 2003年至2008年,連續6年通過病毒分離和(或)間接免疫熒光法,對收集的首都兒科研究所就診的急性呼吸道感染患兒的17 941份標本進行ADV檢測,對其中285份毒株或暘性標本提取DNA,通過3、7、11和21型4對分型引物的多重聚閤酶鏈反應,確定3、7、11和21型ADV,對少數擴增陰性的標本或毒株,再使用2對可擴增所有ADV的通用引物對其DNA進行擴增,將PCR產物直接測序,結果與GenBank上的序列比較,確定其型彆.結果 在17 941份呼吸道標本中,經病毒分離和(或)間接免疫熒光法確定為ADV暘性的標本304份,總暘性率為1.69%.對其中285份毒株或暘性標本的PCR分型結果:272例用3、7、11、21型分型引物擴增確定瞭型彆:3型ADV 174例,佔61.1%(174/285),7型ADV 92例,佔32.3%,11型ADV 6例,佔2.1%;有13例經分型引物擴增為陰性,經過通用引物擴增後的PCR產物測序確定為2型ADV 9例,佔3.2%(9/285);6型ADV2例,佔0.7%,1型和5型ADV各1例,各佔0.4%.結論 本組ADV暘性檢齣率為1.69%.近年來北京地區ADV感染以3、7型為主,其次為2型和11型,1、5和6型較為少見,尚未髮現其他型彆.
목적 료해근년북경지구인과급성호흡도감염환인중인선병독(ADV)감염상황급ADV적형별.방법 2003년지2008년,련속6년통과병독분리화(혹)간접면역형광법,대수집적수도인과연구소취진적급성호흡도감염환인적17 941빈표본진행ADV검측,대기중285빈독주혹양성표본제취DNA,통과3、7、11화21형4대분형인물적다중취합매련반응,학정3、7、11화21형ADV,대소수확증음성적표본혹독주,재사용2대가확증소유ADV적통용인물대기DNA진행확증,장PCR산물직접측서,결과여GenBank상적서렬비교,학정기형별.결과 재17 941빈호흡도표본중,경병독분리화(혹)간접면역형광법학정위ADV양성적표본304빈,총양성솔위1.69%.대기중285빈독주혹양성표본적PCR분형결과:272례용3、7、11、21형분형인물확증학정료형별:3형ADV 174례,점61.1%(174/285),7형ADV 92례,점32.3%,11형ADV 6례,점2.1%;유13례경분형인물확증위음성,경과통용인물확증후적PCR산물측서학정위2형ADV 9례,점3.2%(9/285);6형ADV2례,점0.7%,1형화5형ADV각1례,각점0.4%.결론 본조ADV양성검출솔위1.69%.근년래북경지구ADV감염이3、7형위주,기차위2형화11형,1、5화6형교위소견,상미발현기타형별.
Objective Adenovirus (ADV) is one of the most common causes of acute respiratory infections in infants and children. The objective of this study was to investigate the prevalence of adenovirus infection among pediatric patients with acute respiratory infections in Beijing and the types of the adenoviruses circulating in Beijing on the molecular bases. Method Clinical specimens including throat swabs from outpatients and nasopharyngeal aspirates from hospitalized patients were collected from patients with acute respiratory infections in a consecutive period of 6 years from Jan 2003 to Dec 2008. Adenoviruses were identified from the collected clinical specimens by tissue culture and/or immunofluorescence assay and typed by nested-PCR based on the sequence of the encoding gene of hexon. Primers were designed for PCR amplification using hexon gene of adenovirus as target. One primer pair was designed as universal primers for amplifying a 1278 bp gene fragment located at the hexon gene of adenovirus types 3, 7, 11 and 21. Four primer pairs with the sequences located within the region of this 1278 bp fragment were designed specifically for amplifying adenoviruses types 3, 7, 11 or 21, respectively, which were used for a multiplex nest-PCR in a single tube. The products from this multiplex nest-PCR were 502 bp (for type 3), 311 bp (for type 7),880 bp ( for type 11 ) and 237 bp ( for type 21 ), respectively, and the type of the adenovirus tested can be determined after agarose electrophoresis analysis of the PCR products. For those strains which could not be typed by the multiplex nest-PCR, the gene fragment was amplified by a universal primer pair for all adenovirus types from group A to F and the PCR products were sequenced directly. Result Out of 17 941clinical specimens collected, including 4378 throat swabs from outpatients and 13 563 nasopharyngeal aspirates from hospitalized patients, 304 were adenovirus positive by tissue culture and/or immunofluorescence assay, the overall positive rate was 1.69% (304/179 41 ). Among these 304adenovirus positive specimens, 184 were by virus isolation and 184 by immunofluorescence assay, among which 64 were positive by both methods. The types of the adenoviruses were tested for 285 patients including 174 viral isolates and 111 clinical specimens. By using the multiplex nest-PCR, 272 were typable, including 174 (61.1%, 174/285) for ADV3, 92 (32.3%, 92/285) for ADV7, 6 for ADV11 (2.1%, 6/285) and no adenovirus type 21 was detected. Sequence analysis for those 13 nontypable specimens by the multiplex nest-PCR showed that 9 were ADV2 (3.2%, 9/285 ), 2 were ADV6 (0.7%, 2/285 ), 1 was ADV1(0.4%, 1/285 ) and 1 was ADVS(0.4%, 1/285 ). Most of the patients positive for adenovirus were under 5 years of age and 64.4% were from patients with lower respiratory infections, such as bronchiolitis and pneumonia. All the 5 cases of severe pneumonia with pulmonary failure were caused by ADV7 infection.Conclusion Adenovirus is still an important pathogen for acute respiratory infection in infants and young children and most of the adenoviruses associated with acute respiratory infections in children in Beijing from 2003 to 2008 were ADV3 and ADV7. ADV7 could cause severe lower respiratory infections.
