CAJ | 학술논문

目的探讨KLF样转录因子8(KLF8)在肾癌组织中的表达及其临床意义.方法用RT-PCR和免疫组织化学分别检测肾癌细胞株786-0、OS-RC-2、37例肾癌组织和癌旁正常组织中KLF8 mRNA和蛋白表达情况.结果 RT-PCR显示肾癌细胞株786-0、OS-RC-2 KLF8 mRNA 高表达;肾癌组织为0.80±0.28,正常组织为0.41±0.11,2组间比较差异有统计学意义(P＜0.01).免疫组织化学显示肾癌细胞株中KLF8蛋白高表达;肾癌组织表达(++)～(+++)者17例,癌旁正常组织表达(-)～(+)者29例,2组间比较差异有统计学意义(P＜0.01).KLF8 mRNA和蛋白表达与肿瘤大小、细胞分化程度、临床分期呈正相关,与年龄、性别无相关性.结论肾癌组织KLF8 mRNA和蛋白表达增高,可能与肾癌的发生、进展相关.
목적 탐토KLF양전록인자8(KLF8)재신암조직중적표체급기림상의의.방법 용RT-PCR화면역조직화학분별검측신암세포주786-0、OS-RC-2、37례신암조직화암방정상조직중KLF8 mRNA화단백표체정황.결과 RT-PCR현시신암세포주786-0、OS-RC-2 KLF8 mRNA 고표체;신암조직위0.80±0.28,정상조직위0.41±0.11,2조간비교차이유통계학의의(P＜0.01).면역조직화학현시신암세포주중KLF8단백고표체;신암조직표체(++)～(+++)자17례,암방정상조직표체(-)～(+)자29례,2조간비교차이유통계학의의(P＜0.01).KLF8 mRNA화단백표체여종류대소、세포분화정도、림상분기정정상관,여년령、성별무상관성.결론 신암조직KLF8 mRNA화단백표체증고,가능여신암적발생、진전상관.
Objective To investigate the expression of Kruppel like factor 8(KLF8)in human renal cell carcinoma(RCC)specimens and clinical significance.Methods RT-PCR and immunohistochemistry were used to detect the KLF8 mRNA and protein expression respectively in RCC cell strain 786-0,OS-RC-2 with 37 RCC specimens and adjacent normal tissues of the same patients.Results The expression of KLF8 mRNA was high in RCC cell strain 786-0,OS-RC-2,was 0.80±0.28 and 0.41±0.11 in the RCC specimens and normal tissues respectively(P＜0.01).The level of KLF8 protein expression of 37 RCC specimens(17 was expressed at++-+++)was significantly higher than that of the normal tissues(29 was expressed at--+)(P＜0.01).KLF protein was high expressed in RCC cell strain 786-0,OS-RC-2.The levels of KLF8 mRNA and protein expression were positively correlated with the tumor size,cell differentiation,clinical stage,but not correlated with the patients sex and age.Conclusions The KLF8 mRNA and protein expressions are elevated in the human RCC.KLF8 may be involved in the development and progression of RCC.