CAJ | 학술논문

目的探讨慢性HBV感染母亲血清HBV DNA载量及新生儿外周血中游离母亲DNA与新生儿HBV感染的关系.方法等位基因特异性PCR及半套式PCR技术检测慢性HBV感染母亲及其新生儿外周血中游离母亲DNA.荧光定量PCR对母亲外周血血清中HBV DNA定量检测.数据采用阳性率、X2检验、Fisher精确概率等进行统计学分析.结果筛选出36对母儿信息病例对,有26个新生儿外周血中检测到游离母亲DNA,占72.2%.母-胎DNA转移与新生儿HBsAg、HBV DNA阳性均无关(Fisher精确概率分别为0.278、1.000,均P>0.05),而与新生儿外周血单个核细胞(PBMC)HBV感染有关(Fisher精确概率＝0.026,P<0.05).母-胎DNA转移与母亲血清HBV DNA载量无关(X2=2.097,P>0.05),随着母亲血清HBV DNA载量的增加,其发生新生儿血清HBV DNA阳性的危险性呈增高趋势(总X2=62.21,P<0.05;趋势X2=58.46,P<0.05),而母亲血清HBV DNA载量与新生儿PBMC HBV DNA阳性无关(总X2=4.82,P>0.05).结论母胎DNA转移与新生儿PBMC HBV感染有关,可能是新生儿HBV感染的一个因素.随着母亲HBV DNA载量增高,新生儿血清HBV DNA阳性的危险性越高.
목적 탐토만성HBV감염모친혈청HBV DNA재량급신생인외주혈중유리모친DNA여신생인HBV감염적관계.방법 등위기인특이성PCR급반투식PCR기술검측만성HBV감염모친급기신생인외주혈중유리모친DNA.형광정량PCR대모친외주혈혈청중HBV DNA정량검측.수거채용양성솔、X2검험、Fisher정학개솔등진행통계학분석.결과 사선출36대모인신식병례대,유26개신생인외주혈중검측도유리모친DNA,점72.2%.모-태DNA전이여신생인HBsAg、HBV DNA양성균무관(Fisher정학개솔분별위0.278、1.000,균P>0.05),이여신생인외주혈단개핵세포(PBMC)HBV감염유관(Fisher정학개솔＝0.026,P<0.05).모-태DNA전이여모친혈청HBV DNA재량무관(X2=2.097,P>0.05),수착모친혈청HBV DNA재량적증가,기발생신생인혈청HBV DNA양성적위험성정증고추세(총X2=62.21,P<0.05;추세X2=58.46,P<0.05),이모친혈청HBV DNA재량여신생인PBMC HBV DNA양성무관(총X2=4.82,P>0.05).결론 모태DNA전이여신생인PBMC HBV감염유관,가능시신생인HBV감염적일개인소.수착모친HBV DNA재량증고,신생인혈청HBV DNA양성적위험성월고.
Objective To study the relationships between serum hepatitis B virus (HBV) DNA level in chronic HBV infected mothers, free maternal DNA in newborns' peripheral blood and HBV infection of newborns. Methods Free maternal DNA in newborns' peripheral blood was amplified by allele-specific polymerase chain reaction (As-PCR) and heminested polymerase chain reaction (heminPCR). Serum HBV DNA of pregnant women were detected by fluorescence quantitative real-time PCR. The relationships between mothers' serum HBV DNA level, mother-to-fetus DNA transfer and newborns HBV infection were analyzed by SPSS 13. 0 software. Results Thirty-six pairs of motherfetus informative cases were selected and free maternal DNA in the peripheral blood was detected in 26newborns (72. 2%). Statistical analysis indicated that mother-to-fetus DNA transfer was not related with HBsAg, HBV DNA DOsitive in newborns (Fisher exact Drobabilities were 0. 278 and 1.000,respectively; both P > 0. 05), while it was related with HBV infection in the peripheral bloodmononuclear cell (PBMC) of newborns (Fisher exact probability was 0. 026, P<0. 05). Freematernal DNA transfer was not related with mother HBV DNA level (X2 = 2. 097, P>0. 05). Therisk of HBV DNA positive in newborns increased with mother serum HBV DNA increasing ( total X= 62. 21, P<0. 05; tendency X2 =58. 46, P<0. 05). There was no relationship between motherserum HBV DNA level and PBMC HBV DNA positive in newborns (total X2 =4. 82, P>0. 05).Conclusions DNA transfer from HBV infected mother to fetus is related with PBMC HBV infection innewborns, which could be a risk factor of HBV infection in newborns. The risk of serum HBV DNApositive in newborns increases with mother serum HBV DNA level increasing.