中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
9期
820-824
,共5页
黄芳%邓瑛%曲梅%刘桂荣%刘园%张新%李洁%严寒秋%高志勇%刘白薇%李锡太%黎新宇
黃芳%鄧瑛%麯梅%劉桂榮%劉園%張新%李潔%嚴寒鞦%高誌勇%劉白薇%李錫太%黎新宇
황방%산영%곡매%류계영%류완%장신%리길%엄한추%고지용%류백미%리석태%려신우
腹泻%细菌感染%哨点监测
腹瀉%細菌感染%哨點鑑測
복사%세균감염%초점감측
Diarrhea%Bacterial infections%Sentinel surveillance
目的 了解2010年北京市感染性腹泻致病菌的病原构成、流行特征及主要致病菌的血清型分布。方法 收集2010年4—12月期间北京监测哨点的肠道门诊腹泻病例的粪便或肛拭子标本2118份，采用分离培养、生化鉴定和血清分型的方法对其进行霍乱弧菌、副溶血性弧菌、沙门菌、志贺菌和肠致泻性大肠杆菌等多种肠道致病菌的检测，并对其时间分布、感染者年龄分布及血清型别构成进行分析。结果在2118份标本中，共分离到478株菌，检出率为22.6％。其中志贺菌位居阳性检出菌的首位，占40.8％( 195/478)，副溶血性弧菌、沙门菌和肠致泻性大肠杆菌分别占23.8％(114/478)、19.0％(91/478)和4.8％(23/478)。肠道致病菌的人群分布主要集中在20 ～ 39岁的成年组，其中30 ～39岁检出率最高，检出率为27.2％(92/338)。不同病原菌的人群年龄分布不同。病原菌的检出具有明显季节性，7-10月检出率较高，检出率分别为23.5％( 114/486)、32.8％(176/536)、36.1％ (90/249)、25.9％(29/112)，其余月份检出率＜16.O％。检出的志贺菌以宋内志贺菌为优势血清型，占83.1％(162/195)；副溶血性弧菌以O3∶K6为优势血清型，占63.2％ (72/114)。沙门菌以肠炎和鼠伤寒沙门菌为优势血清型，分别占13.2％( 12/91)、12.1％(11/91)。肠致泻性大肠杆菌以肠致病性大肠杆菌、肠产毒性大肠杆菌2种菌群为主，分别占69.6％( 16/23)、30.4％(7/23)。结论北京市感染性腹泻主要致病菌依次为志贺菌、副溶血性弧菌和沙门菌等。其中志贺菌和沙门菌血清型构成发生了变迁。
目的 瞭解2010年北京市感染性腹瀉緻病菌的病原構成、流行特徵及主要緻病菌的血清型分佈。方法 收集2010年4—12月期間北京鑑測哨點的腸道門診腹瀉病例的糞便或肛拭子標本2118份，採用分離培養、生化鑒定和血清分型的方法對其進行霍亂弧菌、副溶血性弧菌、沙門菌、誌賀菌和腸緻瀉性大腸桿菌等多種腸道緻病菌的檢測，併對其時間分佈、感染者年齡分佈及血清型彆構成進行分析。結果在2118份標本中，共分離到478株菌，檢齣率為22.6％。其中誌賀菌位居暘性檢齣菌的首位，佔40.8％( 195/478)，副溶血性弧菌、沙門菌和腸緻瀉性大腸桿菌分彆佔23.8％(114/478)、19.0％(91/478)和4.8％(23/478)。腸道緻病菌的人群分佈主要集中在20 ～ 39歲的成年組，其中30 ～39歲檢齣率最高，檢齣率為27.2％(92/338)。不同病原菌的人群年齡分佈不同。病原菌的檢齣具有明顯季節性，7-10月檢齣率較高，檢齣率分彆為23.5％( 114/486)、32.8％(176/536)、36.1％ (90/249)、25.9％(29/112)，其餘月份檢齣率＜16.O％。檢齣的誌賀菌以宋內誌賀菌為優勢血清型，佔83.1％(162/195)；副溶血性弧菌以O3∶K6為優勢血清型，佔63.2％ (72/114)。沙門菌以腸炎和鼠傷寒沙門菌為優勢血清型，分彆佔13.2％( 12/91)、12.1％(11/91)。腸緻瀉性大腸桿菌以腸緻病性大腸桿菌、腸產毒性大腸桿菌2種菌群為主，分彆佔69.6％( 16/23)、30.4％(7/23)。結論北京市感染性腹瀉主要緻病菌依次為誌賀菌、副溶血性弧菌和沙門菌等。其中誌賀菌和沙門菌血清型構成髮生瞭變遷。
목적 료해2010년북경시감염성복사치병균적병원구성、류행특정급주요치병균적혈청형분포。방법 수집2010년4—12월기간북경감측초점적장도문진복사병례적분편혹항식자표본2118빈，채용분리배양、생화감정화혈청분형적방법대기진행곽란호균、부용혈성호균、사문균、지하균화장치사성대장간균등다충장도치병균적검측，병대기시간분포、감염자년령분포급혈청형별구성진행분석。결과재2118빈표본중，공분리도478주균，검출솔위22.6％。기중지하균위거양성검출균적수위，점40.8％( 195/478)，부용혈성호균、사문균화장치사성대장간균분별점23.8％(114/478)、19.0％(91/478)화4.8％(23/478)。장도치병균적인군분포주요집중재20 ～ 39세적성년조，기중30 ～39세검출솔최고，검출솔위27.2％(92/338)。불동병원균적인군년령분포불동。병원균적검출구유명현계절성，7-10월검출솔교고，검출솔분별위23.5％( 114/486)、32.8％(176/536)、36.1％ (90/249)、25.9％(29/112)，기여월빈검출솔＜16.O％。검출적지하균이송내지하균위우세혈청형，점83.1％(162/195)；부용혈성호균이O3∶K6위우세혈청형，점63.2％ (72/114)。사문균이장염화서상한사문균위우세혈청형，분별점13.2％( 12/91)、12.1％(11/91)。장치사성대장간균이장치병성대장간균、장산독성대장간균2충균군위주，분별점69.6％( 16/23)、30.4％(7/23)。결론북경시감염성복사주요치병균의차위지하균、부용혈성호균화사문균등。기중지하균화사문균혈청형구성발생료변천。
Objective To explore the pathogenic form,epidemic features and serotype distribution of the pathogenic bacteria causing infectious diarrhea in Beijing. Methods A total of 2118 samples of rectal swabs and stool specimens of diarrheal patients were collected from 6 surveillant intestinal tract clinics during the period between April and October, 2010. Enteric multiple pathogens including Vibrio cholerae, Vibrio parahaemolyticus, Salmonella, Shigella and diarrheagenic Escherichia coli were detected by the isolation culture,biochemical identification and serotyping methods. The population distribution, temperal distribution and serotype distribution of the above pathogenic bacteria were analyzed by descriptive statistical methods. Results 478 strains isolated from the total 2118 specimens were positive for pathogen detection,accounting to 22.6％. Among the 478 strains of pathogenic bacteria, Shigella accounting for 40.8％(195/478) was the most frequent pathogen, followed by Vibrio parahaemolyticus accouting for 23.8％( 114/478 ), Salmonella accounting for 19.0％ ( 91/478 ) and diarrheagenic Escherichia coli accounting for 4.8％ (23/478). Enteric pathogenic bacteria spread mainly among adults aging between 20 and 39; and the distribution was different among different age groups, while the highest detected rate was in 30 - 39 age group,accounting for 27.2％ (92/338). The detected rate of pathogenic bacteria showed evident seasonal variations,with a peak from July to October, whose detected rates were 23.5％ (114/486), 32.8％(176/536) ,36.1 ％ (90/249) and 25.9％ ( 29/112 ) respectively. The detected rates in other months were all under 16.0％. Shigella Sonnei was the dominant serotype,accounting for 83.1％ (162/195). O3∶K6 was the dominant serotype among Vibrio parahaemolyticus, accounting for 63.2％ ( 72/114 ) . Salmonella Enteritidis and Salmonella Typhimurium were dominant serotypes among Salmonella, accounting for 13.2％ (12/91) and 12.1％ (11/91) separately. Enterpathogenic Escherichia coli and enterotoxigenic Escherichia coli were the dominant serotypes among Diarrheagenic Escherichia coli,accounting for 69.6％ (16/23) and 30.4％ (7/23) respectively. ConclusionThe three main pathogenic bacteria causing infectious diarrhea in Beijing are Shigella, Vibrio parahaemolyticus, Salmonella; and there are obvious changes in the serotype distribution of Shigella and Samonella compared to previous years.
