CAJ | 학술논문

目的通过体外试验研究雷公藤内酯醇(triptolide)对浆细胞样树突状细胞(plasmacytoid dendritic cell,pDC)功能及成熟的影响,为进一步阐明雷公藤内酯醇的免疫学活性提供依据.方法从健康志愿者外周血分离单个核细胞,流式细胞仪分选pDC,加入0、5、10、30 μg/L的雷公藤内酯醇共孵育,24 h后收集上清液,ELISA检测pDC分泌的IFN-α、IL-6、TNF-α量,5 d后收集细胞,流式细胞仪检测树突状细胞表型CD11c、CD80、CD86阳性率,光镜观察DC的形态,扫描电镜观察DC的超微结构.结果雷公藤内酯醇显著降低pDC分泌的IFN-α、IL-6、TNF-α,并呈雷公藤内酯醇浓度依赖性(P＜0.05);雷公藤内酯醇可抑制pDC向DC的分化和成熟,并呈雷公藤内酯醇浓度依赖性(P＜0.05).结论雷公藤内酯醇能够降低pDC的功能,并抑制其向DC的分化和成熟.
목적 통과체외시험연구뢰공등내지순(triptolide)대장세포양수돌상세포(plasmacytoid dendritic cell,pDC)공능급성숙적영향,위진일보천명뢰공등내지순적면역학활성제공의거.방법 종건강지원자외주혈분리단개핵세포,류식세포의분선pDC,가입0、5、10、30 μg/L적뢰공등내지순공부육,24 h후수집상청액,ELISA검측pDC분비적IFN-α、IL-6、TNF-α량,5 d후수집세포,류식세포의검측수돌상세포표형CD11c、CD80、CD86양성솔,광경관찰DC적형태,소묘전경관찰DC적초미결구.결과 뢰공등내지순현저강저pDC분비적IFN-α、IL-6、TNF-α,병정뢰공등내지순농도의뢰성(P＜0.05);뢰공등내지순가억제pDC향DC적분화화성숙,병정뢰공등내지순농도의뢰성(P＜0.05).결론 뢰공등내지순능구강저pDC적공능,병억제기향DC적분화화성숙.
Objective To explore the mechanism of immunomodulatory activity of triptolide on healthy volunteers peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs). Methods Healthy volunteers-derived pDCs were sorted by flow cytometry, then incubated with triptolide (0, 5, 10, 30 μg/L). After 24 hours, we detected the concentration of IFN-α, IL-6, TNF-α using ELISA. After 5 days, the cultrural cells were collected and analyzed by flow cytometry, light microscope and electron microscope scanning. Results Triptolide-treated pDCs secreted lower level of IFN-α,IL-6 ,TNF-α, triptolide could inhibit pDCs differentiation to DCs which displayed more immature morphology and immunophenotypes than untreated-pDCs. Conclusion Triptolide could decrease the immune function of pDCs, inhibit differentiation and maturation of pDCs.