中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2011年
3期
176-178
,共3页
肠道病毒属%自噬%3-甲基腺嘌呤%病毒滴度
腸道病毒屬%自噬%3-甲基腺嘌呤%病毒滴度
장도병독속%자서%3-갑기선표령%병독적도
Enterovirus%Autophagy%3-methyladenine%Virus titer
目的 通过研究肠道病毒71型(Enterovirus 71,EV71)感染引起细胞的自噬及抑制自噬对病毒滴度的影响,为进一步明确EV71的致病机制提供基础.方法 利用Western Blot检测EV71感染后RD-A细胞内源性LC3的型别转换和P62的降解来指示细胞发生自噬的水平,通过测定染毒细胞培养上清中EV71病毒CCID50来观察抑制自噬后细胞释放EV71感染性病毒颗粒的变化.结果 EV71的感染促进了细胞LC3的型别转换和F62的降解,诱导了细胞发生自噬;3-MA抑制细胞自噬后,EV71染毒细胞产生的感染性EV71病毒颗粒数量减少.结论 EV71可以诱导细胞发生自噬,细胞自噬可能促进EV71感染性病毒颗粒的产生和释放.
目的 通過研究腸道病毒71型(Enterovirus 71,EV71)感染引起細胞的自噬及抑製自噬對病毒滴度的影響,為進一步明確EV71的緻病機製提供基礎.方法 利用Western Blot檢測EV71感染後RD-A細胞內源性LC3的型彆轉換和P62的降解來指示細胞髮生自噬的水平,通過測定染毒細胞培養上清中EV71病毒CCID50來觀察抑製自噬後細胞釋放EV71感染性病毒顆粒的變化.結果 EV71的感染促進瞭細胞LC3的型彆轉換和F62的降解,誘導瞭細胞髮生自噬;3-MA抑製細胞自噬後,EV71染毒細胞產生的感染性EV71病毒顆粒數量減少.結論 EV71可以誘導細胞髮生自噬,細胞自噬可能促進EV71感染性病毒顆粒的產生和釋放.
목적 통과연구장도병독71형(Enterovirus 71,EV71)감염인기세포적자서급억제자서대병독적도적영향,위진일보명학EV71적치병궤제제공기출.방법 이용Western Blot검측EV71감염후RD-A세포내원성LC3적형별전환화P62적강해래지시세포발생자서적수평,통과측정염독세포배양상청중EV71병독CCID50래관찰억제자서후세포석방EV71감염성병독과립적변화.결과 EV71적감염촉진료세포LC3적형별전환화F62적강해,유도료세포발생자서;3-MA억제세포자서후,EV71염독세포산생적감염성EV71병독과립수량감소.결론 EV71가이유도세포발생자서,세포자서가능촉진EV71감염성병독과립적산생화석방.
Objective To determine whether or not enterovirus 71 ( enteroviurs 71, EV71) may induce autophagy and affect the production and release of EV71 after the treatment of autophagy inhibitor. Methods Western blots were performed to examine the conversion of LC3- Ⅰ to LC3-Ⅱ and the degradation of P62 after the RD-A cells were infected with EV71. CCID50 was determined by checking the virus titer in the supernatant of cells that treated with autophagy inhibitor 3-MA. Results EV71 infection enhances the type conversion of LC3 and degradation of P62. The infectious virus particles were decreased after the treatment of 3-MA. Conclusion EV71 infection could induce cell autophagy and the autophagy might contribute to the production and release of infectious EV71 particles.
