中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2009年
1期
20-23
,共4页
子(癎)前期%硫氧还蛋白%硫氧还蛋白还原酶%氧化应激
子(癎)前期%硫氧還蛋白%硫氧還蛋白還原酶%氧化應激
자(간)전기%류양환단백%류양환단백환원매%양화응격
Preeclampsia%Thioredoxin%Thioredoxin reductase%Oxidative stress
目的 研究子(癎)前期孕妇胎盘硫氧还蛋白还原酶(thioredoxin reductase,TR)、硫氧还蛋白(thioredoxin,Trx)mRNA的表达情况和胎盘TR的活性,探讨Trx系统与子(癎)前期发病的关系.方法 用半定量逆转录-聚合酶链反应检测26例子(癎)前期孕妇(10例轻度子(癎)前期,16例重度子(癎)前期)和28例正常妊娠妇女(对照组)胎盘TR、Trx mRNA表达的情况.采用紫外分光光度计测定胎盘TR活性.结果 (1)子(癎)前期组胎盘TR mRNA(轻度:0.865±0.018,重度0.800±0.025)和Trx mRNA表达(轻度:0.873±0.008,重度0.818±0.025)均低于对照组(TR:0.893±0.023,Trx:0.918±0.023;P均＜0.05),重度子(癎)前期组的表达水平低于轻度子(癎)前期组(P均＜0.05);(2)子(癎)前期组胎盘TR活性分别低于对照组(轻度:17.732±1.653,重度:15.626±1.543,对照:19.770±2.432;P＜0.05),重度子(癎)前期组低于轻度子(癎)前期组(P＜0.05);(3)两组胎盘TR活性和TR mRNA表达呈正相关(r=0.612,P＜0.001).结论 Trx系统的表达异常可能与子(癎)前期的发病有关,并有重要作用.
目的 研究子(癎)前期孕婦胎盤硫氧還蛋白還原酶(thioredoxin reductase,TR)、硫氧還蛋白(thioredoxin,Trx)mRNA的錶達情況和胎盤TR的活性,探討Trx繫統與子(癎)前期髮病的關繫.方法 用半定量逆轉錄-聚閤酶鏈反應檢測26例子(癎)前期孕婦(10例輕度子(癎)前期,16例重度子(癎)前期)和28例正常妊娠婦女(對照組)胎盤TR、Trx mRNA錶達的情況.採用紫外分光光度計測定胎盤TR活性.結果 (1)子(癎)前期組胎盤TR mRNA(輕度:0.865±0.018,重度0.800±0.025)和Trx mRNA錶達(輕度:0.873±0.008,重度0.818±0.025)均低于對照組(TR:0.893±0.023,Trx:0.918±0.023;P均＜0.05),重度子(癎)前期組的錶達水平低于輕度子(癎)前期組(P均＜0.05);(2)子(癎)前期組胎盤TR活性分彆低于對照組(輕度:17.732±1.653,重度:15.626±1.543,對照:19.770±2.432;P＜0.05),重度子(癎)前期組低于輕度子(癎)前期組(P＜0.05);(3)兩組胎盤TR活性和TR mRNA錶達呈正相關(r=0.612,P＜0.001).結論 Trx繫統的錶達異常可能與子(癎)前期的髮病有關,併有重要作用.
목적 연구자(간)전기잉부태반류양환단백환원매(thioredoxin reductase,TR)、류양환단백(thioredoxin,Trx)mRNA적표체정황화태반TR적활성,탐토Trx계통여자(간)전기발병적관계.방법 용반정량역전록-취합매련반응검측26례자(간)전기잉부(10례경도자(간)전기,16례중도자(간)전기)화28례정상임신부녀(대조조)태반TR、Trx mRNA표체적정황.채용자외분광광도계측정태반TR활성.결과 (1)자(간)전기조태반TR mRNA(경도:0.865±0.018,중도0.800±0.025)화Trx mRNA표체(경도:0.873±0.008,중도0.818±0.025)균저우대조조(TR:0.893±0.023,Trx:0.918±0.023;P균＜0.05),중도자(간)전기조적표체수평저우경도자(간)전기조(P균＜0.05);(2)자(간)전기조태반TR활성분별저우대조조(경도:17.732±1.653,중도:15.626±1.543,대조:19.770±2.432;P＜0.05),중도자(간)전기조저우경도자(간)전기조(P＜0.05);(3)량조태반TR활성화TR mRNA표체정정상관(r=0.612,P＜0.001).결론 Trx계통적표체이상가능여자(간)전기적발병유관,병유중요작용.
Objective To assess the expression of thioredoxin reductase(TR)and thioredoxin (Trx)mRNA,TR activity in human placenta in women with preeclampsia,and to discuss the relationship between Trx system and the pathogenesis of preeclampsia.Methods Twenty-sixwomen with preeclampsia(10 mild and 16 severe)and 28 healthy gravidas (control) were included in this study.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR)were employed to demonstrate the expression of TR and Trx in placentas.TR activity was measured spectrophotometrically.Results The expression of TR mRNA(mild:0.865±0.018;severe:0.800±0.025,P＜0.05)and Trx mRNA(mild:0.873±0.008;severe:0.818±0.025,P＜0.05)were lower in placenta of preeclampsia than in the control group(TR mRNA:0.893±0.023;Trx mRNA:0.918±0.023.P＜0.05),and those levels in the severe preeclampsia group were lower than in the mild ones.Both the mild and severe preeclampsia groups showed lower TR activity than the control(mild:17.732±1.653;severe:15.626±1.543 vs 19.770±2.432,P＜0.05),and that of the severe preeclampsia group was lower than that of the mild ones.Positive correlation was found between the TR activity and TR mRNA expression in both groups(r=0.612,P＜0.001).Conclusions Abnormal decreased expression of TR mRNA and Trx mRNA and TR activity in human placenta of preeclampsia might participate and play an important role in the pathogenesis of preeclampsia.
