CAJ | 학술논문

目的观察乳腺肿瘤细胞经血卟啉单甲醚介导的光动力学疗法( HMME-PDT)作用后白细胞介素(IL )-2和IL-6的变化.方法常规传代培养乳腺癌Bcap-37细胞,取对数生长期的细胞,按空白对照组、实验组(激光照射组、光敏剂组、综合组)进行HMME-PDT作用,采用放射免疫法检测不同时间点细胞IL-2、IL-6的含量变化.结果在HMME-PDT作用后,IL-2含量随时间延长增加,综合组在12、24和48 h与对照组、激光照射组、光敏剂组比较差异有统计学意义(P＜0.05).IL-6的含量随时间延长降低,在12、24h变化最明显,综合组与对照组、激光照射组和光敏剂组比较差异均有统计学意义(P＜0.05).结论 HMME-PDT可能通过改变IL-2、IL-6的活性对乳腺肿瘤细胞产生杀伤作用,为临床患者调节免疫功能和辅助诊断提供客观指标.
목적 관찰유선종류세포경혈계람단갑미개도적광동역학요법( HMME-PDT)작용후백세포개소(IL )-2화IL-6적변화.방법 상규전대배양유선암Bcap-37세포,취대수생장기적세포,안공백대조조、실험조(격광조사조、광민제조、종합조)진행HMME-PDT작용,채용방사면역법검측불동시간점세포IL-2、IL-6적함량변화.결과 재HMME-PDT작용후,IL-2함량수시간연장증가,종합조재12、24화48 h여대조조、격광조사조、광민제조비교차이유통계학의의(P＜0.05).IL-6적함량수시간연장강저,재12、24h변화최명현,종합조여대조조、격광조사조화광민제조비교차이균유통계학의의(P＜0.05).결론 HMME-PDT가능통과개변IL-2、IL-6적활성대유선종류세포산생살상작용,위림상환자조절면역공능화보조진단제공객관지표.
Objective To observe IL-2 and IL-6 changes in the breast tumor Bcap-37 cells reated by hematoporphyrin nonomethyl ether mediated photodynamic therapy (HMME-PDT).Methods Cells in logarithmic growth phase were collected among breast cancer cells cultured in conventional methods.According to blank control group or the experimental group (laser irradiation group,photosensitive agent group and HMME-PDT group),PDT in addition to HMME and HMME-PDT were conducted.The changes of IL-2,IL-6 were detected by radioimmunoassay.Results After HMME-PDT,IL-2 was increased as time passed.After 12,24 and 48 h,compared with IL-2 level in the control group,in laser irradiation group or photosensitive agent group,the levels of IL-2 in HMME-PDT group was significantly differences (P ＜ 0.05).But IL-6 levels decreased.The most obvious changes of IL-6 levels happened at 12h and 24h.There was significant differences between IL-6 in HMME-PDT group with the control group,laser irradiation group or photosensitive agent group (P ＜ 0.05).Conclusion HMME-PDT maybe have destruction effect by altering IL-2,IL-6 activity on breast tumor cells,which provides objective indicators for clinical patients to regulate immune function and auxiliary diagnosis.