CAJ | 학술논문

当蓝细菌Synechocystis sp. PCC 6803在添加葡萄糖的BG-11培养基中培养时,细胞出现了一种新脂.这种脂经浓硫酸/α-萘酚染色反应证实为糖脂,记为糖脂-x.这一糖脂的出现伴随着其他脂、尤其是双半乳糖甘油二酯含量的下降.此外,在添加果糖、麦芽糖、乳糖等其他碳源的培养基中生长的细胞中也检测到这一糖脂.活性氧猝灭剂硫代硫酸钠加入到培养基中能有效地抑制糖脂x的出现.当在BG-11培养基中加入0.3%硫代硫酸钠后,糖脂x仅能在培养基中添加高浓度(100 mmol/L)的葡萄糖且细胞生长处于后指数期时检测到.这些结果表明蓝细菌Synechocystis sp. PCC 6803细胞在含葡萄糖的培养基中生长出现的一种新糖脂可能与活性氧有关.
당람세균Synechocystis sp. PCC 6803재첨가포도당적BG-11배양기중배양시,세포출현료일충신지.저충지경농류산/α-내분염색반응증실위당지,기위당지-x.저일당지적출현반수착기타지、우기시쌍반유당감유이지함량적하강.차외,재첨가과당、맥아당、유당등기타탄원적배양기중생장적세포중야검측도저일당지.활성양졸멸제류대류산납가입도배양기중능유효지억제당지x적출현.당재BG-11배양기중가입0.3%류대류산납후,당지x부능재배양기중첨가고농도(100 mmol/L)적포도당차세포생장처우후지수기시검측도.저사결과표명람세균Synechocystis sp. PCC 6803세포재함포도당적배양기중생장출현적일충신당지가능여활성양유관.
A novel lipid occurred when cyanobacterium Synechocystis sp. PCC 6803 cells were grown in BG-11 medium with glucose applied. This lipid was determined to be a glycolipid, designated glycolipid-x (Glyco-x), by staining with α-naphthol and concentrated sulfuric acid. The occurrence of Glyco-x accompanies the disappearance of other lipids, especially DGDG. Glyco-x can also be observed in cells grown in BG-11 medium with the application of other carbon sources: fructose, maltose and lactose. Sodium thiosulfate, an effective scavenger of reactive oxygen intermediates, showed strong capability to inhibit glucose-induced occurrence of Glyco-x. In the presence of 0.3% sodium thiosulfate, Glyco-x could only be detected in cells grown in BG-11 medium with 100 mmol/L glucose applied in late-exponential phase. These results suggest that reactive oxygen species might be involved in the occurrence of Glyco-x in cyanobacterium Synechocystis sp. PCC 6803 cells grown in the presence of glucose.