安徽农业科学
安徽農業科學
안휘농업과학
JOURNAL OF ANHUI AGRICULTURAL SCIENCES
2009年
31期
15141-15143,15156
,共4页
石榴%成熟叶片%CTAB法%基因组DNA%提取
石榴%成熟葉片%CTAB法%基因組DNA%提取
석류%성숙협편%CTAB법%기인조DNA%제취
Pomegranate (Punka granatum Linn. )%Mature leaves%CTAB method%Genomic DNA%Extraction
[目的]为了从成熟石榴叶片中提取高质量、高产量的基因组DNA.[方法]针对成熟石榴叶片含多糖、多酚的特性,用改良CTABⅠ及改良CTAB Ⅱ法分别提取2个品种(墨石榴和青皮软籽石榴)的成熟石榴鲜叶、冻叶、干叶基因组DNA,并通过琼脂糖凝胶电泳、紫外分光光度计、酶切鉴定.[结果]改良CTAB Ⅱ法提取的基因组DNA电泳时点样孔干净,无拖带,OD_(260 nm)/OD_(280 nm)为1.7～1.9,酶切完全,其质量、产量都高于改良CTAB Ⅰ法.[结论]改良CTAB Ⅱ法是提取成熟石榴叶片中高质量、高产量基因组DNA的有效方法.
[目的]為瞭從成熟石榴葉片中提取高質量、高產量的基因組DNA.[方法]針對成熟石榴葉片含多糖、多酚的特性,用改良CTABⅠ及改良CTAB Ⅱ法分彆提取2箇品種(墨石榴和青皮軟籽石榴)的成熟石榴鮮葉、凍葉、榦葉基因組DNA,併通過瓊脂糖凝膠電泳、紫外分光光度計、酶切鑒定.[結果]改良CTAB Ⅱ法提取的基因組DNA電泳時點樣孔榦淨,無拖帶,OD_(260 nm)/OD_(280 nm)為1.7～1.9,酶切完全,其質量、產量都高于改良CTAB Ⅰ法.[結論]改良CTAB Ⅱ法是提取成熟石榴葉片中高質量、高產量基因組DNA的有效方法.
[목적]위료종성숙석류협편중제취고질량、고산량적기인조DNA.[방법]침대성숙석류협편함다당、다분적특성,용개량CTABⅠ급개량CTAB Ⅱ법분별제취2개품충(묵석류화청피연자석류)적성숙석류선협、동협、간협기인조DNA,병통과경지당응효전영、자외분광광도계、매절감정.[결과]개량CTAB Ⅱ법제취적기인조DNA전영시점양공간정,무타대,OD_(260 nm)/OD_(280 nm)위1.7～1.9,매절완전,기질량、산량도고우개량CTAB Ⅰ법.[결론]개량CTAB Ⅱ법시제취성숙석류협편중고질량、고산량기인조DNA적유효방법.
[Objective] The research aimed to extract the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves. [ Method ] According to the character of containing amylase and polyphenol, genomic ONA in fresh leaves, frozen leaves and dry leaves of 2 kinds of mature pomegranates were extracted by improved CTAB I and CTAB U method. And they were identified by agarose gel elec-trophoresis,ultraviolet spectrophotometer method and enzyme digestion. [Result] The results showed that the sample hole was clean and no pulling, OD_(260nm)/OD_(280nm) was 1.7-1.9 when the genomic DNA extracted by improved CTAB II method was electrophoresis. And its quality and yield was high than that extracted by improved CTAB I method. [ Conclusion] The improved CTAB H method was the effective method of extracting the high quality and yield genomic DNA from mature pomegranate (Punica granatum Linn. ) leaves.
