CAJ | 학술논문

为提高植物抗寒性,本文构建一个植物表达载体,并对非洲菊进行转化.用已克隆的强抗冷植物兵豆的甘油-3-磷酸转酰酶(GPAT)基因构建表达载体,将GPAT插入表达载体pBI121质粒取代该质粒上原有的GUS基因,并用PCR和酶切进行鉴定.用农杆菌介导的方法将该质粒转化到非洲菊中.结果表明该植物表达载体构建成功,同时对非洲菊高效转化体系进行探索,获得卡那霉素抗性苗7苗.
위제고식물항한성,본문구건일개식물표체재체,병대비주국진행전화.용이극륭적강항랭식물병두적감유-3-린산전선매(GPAT)기인구건표체재체,장GPAT삽입표체재체pBI121질립취대해질립상원유적GUS기인,병용PCR화매절진행감정.용농간균개도적방법장해질립전화도비주국중.결과표명해식물표체재체구건성공,동시대비주국고효전화체계진행탐색,획득잡나매소항성묘7묘.
In order to enhance the chilling resistance of plant, a new plant expression vector was made and transformed into Gerbera hybrida. Glycerol-3-phosphate acyltransferase (GPAT) cDNA obtained from Lens culinaris was cloned into expression vector pBI121 by replacing GUS gene. The recombinant plasmid was confirmed by restriction endonuclease analysis and PCR detection. Agrobacterium method was used to transform the plasmid into Gerbera hybrida. The result shows that the new plant expression vector was constructed successfully. The genetic transformation system of Gerbera hybrida was studied and 7 kanamycin resistant plants were obtained.