中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2009年
6期
452-456
,共5页
张若曦%苑望%伍晓雄%戴汉川
張若晞%苑望%伍曉雄%戴漢川
장약희%원망%오효웅%대한천
共轭亚油酸%高脂饮食%基因%高脂血症%大鼠
共軛亞油痠%高脂飲食%基因%高脂血癥%大鼠
공액아유산%고지음식%기인%고지혈증%대서
Conjugated linoleic acid%High fat diet%Visfatin%Hyperlipidemia%Rat
目的 建立高脂血症大鼠模型,分析共轭亚油酸(CLA)对其脂质代谢和visfatin基因表达水平的影响,为进一步研究CLA对脂肪代谢的调控机制奠定基础.方法 用高脂饲料饲喂雄性Wistar大鼠,4周后断尾采血测定血清甘油三酯(TG)、总胆固醇(CHO)和血糖(GLU)含量,将构建成功的高脂血症大鼠分为实验组和对照组,实验组每天定时灌胃CLA(0.8 mL /0.1 kg),每周定时称重,记录采食量;4周后眼球采血,测定血清中GLU、CHO、TG、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平;断颈处死大鼠,分离体脂并提取肝脏总RNA,半定量RT-PCR分析visfatin基因表达水平.结果 高脂饲料饲喂4周后,模型组大鼠血清TG、CHO、GLU明显高于对照组大鼠(P< 0.05),表明高脂大鼠模型构建成功.灌胃CLA 4周后,实验组大鼠体重、体脂和采食量低于对照组大鼠(P< 0.05),血清中GLU、CHO、TG、LDL含量明显降低,但实验组HDL浓度升高.RT-PCR实验结果 表明,实验组大鼠visfatin基因表达水平明显低于对照组大鼠(P< 0.05).结论 CLA能降低高脂血症大鼠摄食量,改善高血脂大鼠脂质代谢,并能降低visfatin基因的表达水平.
目的 建立高脂血癥大鼠模型,分析共軛亞油痠(CLA)對其脂質代謝和visfatin基因錶達水平的影響,為進一步研究CLA對脂肪代謝的調控機製奠定基礎.方法 用高脂飼料飼餵雄性Wistar大鼠,4週後斷尾採血測定血清甘油三酯(TG)、總膽固醇(CHO)和血糖(GLU)含量,將構建成功的高脂血癥大鼠分為實驗組和對照組,實驗組每天定時灌胃CLA(0.8 mL /0.1 kg),每週定時稱重,記錄採食量;4週後眼毬採血,測定血清中GLU、CHO、TG、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)水平;斷頸處死大鼠,分離體脂併提取肝髒總RNA,半定量RT-PCR分析visfatin基因錶達水平.結果 高脂飼料飼餵4週後,模型組大鼠血清TG、CHO、GLU明顯高于對照組大鼠(P< 0.05),錶明高脂大鼠模型構建成功.灌胃CLA 4週後,實驗組大鼠體重、體脂和採食量低于對照組大鼠(P< 0.05),血清中GLU、CHO、TG、LDL含量明顯降低,但實驗組HDL濃度升高.RT-PCR實驗結果 錶明,實驗組大鼠visfatin基因錶達水平明顯低于對照組大鼠(P< 0.05).結論 CLA能降低高脂血癥大鼠攝食量,改善高血脂大鼠脂質代謝,併能降低visfatin基因的錶達水平.
목적 건립고지혈증대서모형,분석공액아유산(CLA)대기지질대사화visfatin기인표체수평적영향,위진일보연구CLA대지방대사적조공궤제전정기출.방법 용고지사료사위웅성Wistar대서,4주후단미채혈측정혈청감유삼지(TG)、총담고순(CHO)화혈당(GLU)함량,장구건성공적고지혈증대서분위실험조화대조조,실험조매천정시관위CLA(0.8 mL /0.1 kg),매주정시칭중,기록채식량;4주후안구채혈,측정혈청중GLU、CHO、TG、저밀도지단백(LDL)、고밀도지단백(HDL)수평;단경처사대서,분리체지병제취간장총RNA,반정량RT-PCR분석visfatin기인표체수평.결과 고지사료사위4주후,모형조대서혈청TG、CHO、GLU명현고우대조조대서(P< 0.05),표명고지대서모형구건성공.관위CLA 4주후,실험조대서체중、체지화채식량저우대조조대서(P< 0.05),혈청중GLU、CHO、TG、LDL함량명현강저,단실험조HDL농도승고.RT-PCR실험결과 표명,실험조대서visfatin기인표체수평명현저우대조조대서(P< 0.05).결론 CLA능강저고지혈증대서섭식량,개선고혈지대서지질대사,병능강저visfatin기인적표체수평.
Objective To establish a rat model of hyperlipidemia and analyze the effect of conjugated linoleic acid (CLA) on lipid metabolism and expression of visfatin gene. Method High fat diet was used to establish the hyperlipidemia rat model. Blood was taken at four weeks after high fat diet feeding to analyze the level of glucose (GLU), cholesterol (CHO) and triglyeride(TG). The rats were divided into experimental group and control group after the hyperlipidemia rat model was established successfully. The experimental group rats were treated with CLA(0.8 mL /0.1 kg)orally for four weeks. The food intake and body weight were recorded. The rats were sacrificed, and both body fat and serum lipid levels were measured. Semi-quantitative RT-PCR was used to measure the expression level of visfatin mRNA. Result The hyperlipidemic rat models were induced by high fat diet successfully. The body weight, food intake and body fat in the rats of CLA experiment group were significantly decreased compared with that of the control group (P< 0.05). The level of GLU, CHO, TG and LDL in the experimental group were significantly lower than that in the control group (P< 0.05), but the serum HDL-C was increased in the experimental group. Semi-quantitative RT-PCR demonstrated that the expression level of visfatin gene of the experimental group was lower than that in the control group. Conclusion CLA can reduce the expression of visfatin gene and improve the lipid metabolism in hyperlipidemic rats.
