CAJ | 학술논문

目的:探讨力竭运动后不同时相大鼠心肌细胞间粘附因子(ICAM-1)表达的变化特点.方法:100只健康成年雄性SD大鼠,分为一次力竭游泳运动组(n=40)、2周反复力竭游泳运动组(n=40)及相应对照组(n=20),对照组不运动.分别于力竭运动后0、6、12及24小时取材,应用免疫荧光技术和图像分析方法研究大鼠心肌ICAM-1含量的变化.结果:两种力竭运动后,大鼠心肌细胞快速表达,运动后6小时后达到峰值,蛋白含量变化呈先上升后下降的趋势;除反复力竭后24小时组与对照组无显著性差异(P>0.05),其他各时相组与对照组相比均有不同程度升高(P<0.01).比较两种力竭运动后ICAM-1蛋白含量发现,反复力竭后即刻大鼠心肌各部位ICAM-1蛋白含量均高于一次力竭后即刻组(P<0.01),力竭后6小时组各部位之间无显著性差异(P>0.05),12小时和24小时组,一次力竭后大鼠心肌各部位ICAM-1蛋白含量要高于反复力竭(P<0.01).结论:不同力竭运动后心肌细胞ICAM-1水平的升高可以诱导细胞因子的激活,介导心肌细胞的粘附和浸润等炎症反应,构成运动性心肌微损伤的发生机制之一.
목적:탐토력갈운동후불동시상대서심기세포간점부인자(ICAM-1)표체적변화특점.방법:100지건강성년웅성SD대서,분위일차력갈유영운동조(n=40)、2주반복력갈유영운동조(n=40)급상응대조조(n=20),대조조불운동.분별우력갈운동후0、6、12급24소시취재,응용면역형광기술화도상분석방법연구대서심기ICAM-1함량적변화.결과:량충력갈운동후,대서심기세포쾌속표체,운동후6소시후체도봉치,단백함량변화정선상승후하강적추세;제반복력갈후24소시조여대조조무현저성차이(P>0.05),기타각시상조여대조조상비균유불동정도승고(P<0.01).비교량충력갈운동후ICAM-1단백함량발현,반복력갈후즉각대서심기각부위ICAM-1단백함량균고우일차력갈후즉각조(P<0.01),력갈후6소시조각부위지간무현저성차이(P>0.05),12소시화24소시조,일차력갈후대서심기각부위ICAM-1단백함량요고우반복력갈(P<0.01).결론:불동력갈운동후심기세포ICAM-1수평적승고가이유도세포인자적격활,개도심기세포적점부화침윤등염증반응,구성운동성심기미손상적발생궤제지일.
Objective To investigate the change in the content of intracellular adhesion molecule-1(ICAM-1)in myocardium of rats at different time courses after exhausted exercise.Methods One hundred male SD rats were divided into sedentary control group,single bout of exhausted exercise group and 14-day consecutive exhausted exercise group.Rats were killed at 0.6,1 2 and 24 hours after exhausted exercise.Immunofluorescence technique and image analysis method were used to study the dynamic changes in the content of ICAM-1 in myocardium.Results Comparing with the control group,following these 2 modes of exhausted exercise,the content of ICAM-1 in rat myocardium increased and reached its peak value 6 hours after exercise,and then decreased,and ICAM-1 content in all other time courses were significantly increased(P<0.01)after exhaustive exercise,except 24h after exercise group (P>0.05).ICAM-1 content from different parts of myocardium immediately after consecutive exhausted exercise was significantly higher than that after single bout of exhausted exercise (P<0.01),and the differences disappeared 6 hours after exercise(P>0.05).ICAM-1 content in 12-and 24-hour single bout of exhausted exercise groups was significantly higher than that in consecutive exhausted exercise groups(P<0.01).Conclusion The increase of ICAM-1 level in myocardium after difierent modes of exhausted exercise can activate cytokine,mediate inflammatory responses of myocytes,such as adhesion and infiltration,which contribute to the exercise-induced myocardial micro-injury.