CAJ | 학술논문

本文首先对铜配合物催化DNA氧化断裂的机制及相关因素,包括DNA的结合能力与方式、活性氧物种的形成和活性氧物种对底物的损伤等进行了讨论,然后结合本组的工作对配合物的结构及其对DNA切割性能的影响分别进行了总结,探讨了核数、核种类、位阻、电荷、结合方式以及氧化还原电位等因素的影响,同时还对光激发铜配合物断裂DNA的机制和性能进行了分析,最后对特异性含铜DNA断裂试剂的不同设计策略及相关切割行为进行了系统总结,这不仅有利于对铜配合物促进DNA断裂行为的系统理解,而且对于进一步设计高效含铜人工核酸酶以实现DNA的特异性断裂具有良好的指导意义.
본문수선대동배합물최화DNA양화단렬적궤제급상관인소,포괄DNA적결합능력여방식、활성양물충적형성화활성양물충대저물적손상등진행료토론,연후결합본조적공작대배합물적결구급기대DNA절할성능적영향분별진행료총결,탐토료핵수、핵충류、위조、전하、결합방식이급양화환원전위등인소적영향,동시환대광격발동배합물단렬DNA적궤제화성능진행료분석,최후대특이성함동DNA단렬시제적불동설계책략급상관절할행위진행료계통총결,저불부유리우대동배합물촉진DNA단렬행위적계통리해,이차대우진일보설계고효함동인공핵산매이실현DNA적특이성단렬구유량호적지도의의.
The oxidative DNA cleavage promoted by copper complexes and the related factors including the DNA binding mode/ability, the formation of reactive oxygen species (ROS) and the ROS damaging DNA were initially discussed in this review. Then, the copper complex structral factors affected the DNA cleavage, such as the metal center, nuclear number, steric hinderance, charge number, DNA binding mode and redox potential of copper center were surveyed based on the reported works of ours and others. In addition, the photo-induced DNA cleavage behavior of copper complexes was also investigated. Finally, the different strategies of copper nuclease construction for the specific DNA cleavage were systematically discussed. All these should not only favor to understand the oxidative DNA cleavage mediated by copper complexes but also promote to construct novel copper-containing artificical nucleases in the future.