CAJ | 학술논문

目的观察体外无细胞核糖体表达系统筛选得到的、能与细菌膜结合的多肽的体外抑菌活性变化,初步评价该筛选系统的可行性.方法采用核糖体展示系统和细胞膜模型筛选可结合于细胞膜的多肽,对筛选的多肽进行结构预测分析.选择形成α-螺旋结构可能性大、溶解性尚可的多肽(C13)进行合成.利用改良的微量肉汤稀释法测定C13和硫酸庆大霉素对G+(金黄色葡萄球菌)和G-(大肠杆菌、伤寒杆菌)细菌的抗菌活性--最小抑菌浓度(MIC)和最小杀菌浓度(MBC).结果 C13对试验细菌的MIC均为400 mg/L,但在现有浓度范围对试验菌的MBC则未测得.结论体外无细胞核糖体表达系统筛选的抗菌肽C13具有一定的抑菌活性,表明利用人工膜模型和核糖体表达进行抗菌肽筛选的方法是可行的,但有效抗菌肽的筛选需进一步的抑菌试验证实.
목적 관찰체외무세포핵당체표체계통사선득도적、능여세균막결합적다태적체외억균활성변화,초보평개해사선계통적가행성.방법 채용핵당체전시계통화세포막모형사선가결합우세포막적다태,대사선적다태진행결구예측분석.선택형성α-라선결구가능성대、용해성상가적다태(C13)진행합성.이용개량적미량육탕희석법측정C13화류산경대매소대G+(금황색포도구균)화G-(대장간균、상한간균)세균적항균활성--최소억균농도(MIC)화최소살균농도(MBC).결과 C13대시험세균적MIC균위400 mg/L,단재현유농도범위대시험균적MBC칙미측득.결론 체외무세포핵당체표체계통사선적항균태C13구유일정적억균활성,표명이용인공막모형화핵당체표체진행항균태사선적방법시가행적,단유효항균태적사선수진일보적억균시험증실.
Objective To investigate the activity of the peptids that were screened by cell-free ribosome display system and combined with bacterial membrane against bacterium in vitro, and to evaluate the screening system feasibility. Methods The peptid (C13) was synthesized based on the protein structure prediction results that showed C13 with a alpha-helix and good dissolution. In vitro, susceptibilities of C13 and gentamicin sulphate were evaluated against several Gram-positive (Staphylococcus aureus) and Gram-negative bacterium (Escherichia coli, Bacillus typhi), using modified broth microdilution method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were measured. Results The MIC of C13 against all test bacterium was 400 mg/L. The MBC of C13 against the test bacterium was not detected within the concentration range. Conclusions The peptids C13 screened by cell-free ribosome display system have aitimicrobial activity in some content. However it is necessary for discovering the effective antibacterial peptides to validate by against bacterium experiments. This method of peptids screening by artificial membrane and ribosome display is successful.