中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2010年
2期
127-130
,共4页
朱冰%钟家禹%夏慧敏%龚四堂%肖密丝%谢嘉慧%张莹莹%华亮%连广琬
硃冰%鐘傢禹%夏慧敏%龔四堂%肖密絲%謝嘉慧%張瑩瑩%華亮%連廣琬
주빙%종가우%하혜민%공사당%초밀사%사가혜%장형형%화량%련엄완
手足口病%肠道病毒感染%柯萨奇病毒感染%逆转录聚合酶链反应
手足口病%腸道病毒感染%柯薩奇病毒感染%逆轉錄聚閤酶鏈反應
수족구병%장도병독감염%가살기병독감염%역전록취합매련반응
Hand%foot and mouth disease%Enterovirus infection%Coxsackievirus infections%Reverse transcriptase polymerase chain reaction
目的 了解广州地区2008年5～6月份手口足病的病原.方法 设计肠道病毒71型、柯萨奇病毒A16型和肠道病毒属通用实时荧光RT-PCR引物和TaqMan探针,运用TaqMan实时荧光RT-PCR技术,对2008年5至6月,到广州市儿童医院就诊的疑似手足口病患儿咽拭子标本1023例(份),同时分别进行肠道病毒属、肠道病毒71型和柯萨奇病毒A16型检测.对肠道病毒属荧光RT-PCR检测阳性.而肠道病毒71型和柯萨奇病毒A16型荧光RT-PCR检测阴性的标本进行5'非编码区(Untranslated Region UTR)的扩增和序列测定.结果 在1023份临床标本中,肠道病毒属阳性434例,检出率为42.42%.其中,肠道病毒71型276例,柯萨奇病毒A16型126例,肠道病毒84型4份,埃可病毒11型3例,埃可病毒9型2例,柯萨奇病毒B3型3例,柯萨奇病毒A10型4例,柯萨奇病毒A6型3例,柯萨奇病毒A12或A5型6例,另有7例难以区分型别,没有混合感染.结论 2008年5～6月广州地区手足口病的主要病原是肠道病毒71型和柯萨奇病毒A16型,并有肠道病毒84型、柯萨奇病毒A10型、柯萨奇病毒A12型、柯萨奇病毒A6型、柯萨奇病毒B3型、埃可病毒11型、埃可病毒9型等小部分其他型别肠道病毒感染.
目的 瞭解廣州地區2008年5～6月份手口足病的病原.方法 設計腸道病毒71型、柯薩奇病毒A16型和腸道病毒屬通用實時熒光RT-PCR引物和TaqMan探針,運用TaqMan實時熒光RT-PCR技術,對2008年5至6月,到廣州市兒童醫院就診的疑似手足口病患兒嚥拭子標本1023例(份),同時分彆進行腸道病毒屬、腸道病毒71型和柯薩奇病毒A16型檢測.對腸道病毒屬熒光RT-PCR檢測暘性.而腸道病毒71型和柯薩奇病毒A16型熒光RT-PCR檢測陰性的標本進行5'非編碼區(Untranslated Region UTR)的擴增和序列測定.結果 在1023份臨床標本中,腸道病毒屬暘性434例,檢齣率為42.42%.其中,腸道病毒71型276例,柯薩奇病毒A16型126例,腸道病毒84型4份,埃可病毒11型3例,埃可病毒9型2例,柯薩奇病毒B3型3例,柯薩奇病毒A10型4例,柯薩奇病毒A6型3例,柯薩奇病毒A12或A5型6例,另有7例難以區分型彆,沒有混閤感染.結論 2008年5～6月廣州地區手足口病的主要病原是腸道病毒71型和柯薩奇病毒A16型,併有腸道病毒84型、柯薩奇病毒A10型、柯薩奇病毒A12型、柯薩奇病毒A6型、柯薩奇病毒B3型、埃可病毒11型、埃可病毒9型等小部分其他型彆腸道病毒感染.
목적 료해엄주지구2008년5～6월빈수구족병적병원.방법 설계장도병독71형、가살기병독A16형화장도병독속통용실시형광RT-PCR인물화TaqMan탐침,운용TaqMan실시형광RT-PCR기술,대2008년5지6월,도엄주시인동의원취진적의사수족구병환인인식자표본1023례(빈),동시분별진행장도병독속、장도병독71형화가살기병독A16형검측.대장도병독속형광RT-PCR검측양성.이장도병독71형화가살기병독A16형형광RT-PCR검측음성적표본진행5'비편마구(Untranslated Region UTR)적확증화서렬측정.결과 재1023빈림상표본중,장도병독속양성434례,검출솔위42.42%.기중,장도병독71형276례,가살기병독A16형126례,장도병독84형4빈,애가병독11형3례,애가병독9형2례,가살기병독B3형3례,가살기병독A10형4례,가살기병독A6형3례,가살기병독A12혹A5형6례,령유7례난이구분형별,몰유혼합감염.결론 2008년5～6월엄주지구수족구병적주요병원시장도병독71형화가살기병독A16형,병유장도병독84형、가살기병독A10형、가살기병독A12형、가살기병독A6형、가살기병독B3형、애가병독11형、애가병독9형등소부분기타형별장도병독감염.
Objective To understand the etiology of hand, foot and mouth disease (HFMD) in Guangzhou area in 2008. Method Totally 1023 clinical specimens were collected from pediatric patients suspected of HFMD in 2008. TaqMan real-time RT-PCR were used for detection of enterovirus 71 (EV71), Coxsackievims A16 (CA16) and other enteroviruses. The specimens which were entrovirus positive by RT-PCR method with universal primer but EV71 and CA16 negative, were amplified and sequenced for 5' untranslated region. Result Enterovirus was identified from 434 of 1023 samples and detection rate of entervirns was 42.42% ; of the 434 samples, 276 were positive for EV71 ( 63.6% ), 126 for CA16 (29%), 4 samples for enterovirus 84, 3 for Echovirus 11, 2 for Echovims 9, 3 for Coxsackievirus B3, 4 for Coxsackievirus A10, 3 for Coxsackievirus A6, 6 for Coxsackievirus A12 or A5, and for 7 samples typing was difficult. Conclusion The major causative agents of HFMD in Guangzhou were EV71 and CA16 in 2008, and EV84, CA10, CA12, CA6, COSB3, ECHV11, ECHV9 were also the pathogens for smaller proportions of patients.
