CAJ | 학술논문

目的探讨酪氨酸(Tyr)干预对心理应激大鼠中枢多巴胺(DA)含量及其更新率的影响.方法 40只SD大鼠按数字表法随机分为5组:对照组、心理应激组和3个剂量Tyr干预组(分别经饲料每天给予Tyr 250、500、1000 mg/kg),每组8只.建立大鼠Communication Box心理应激模型,采用高效液相色谱-电化学(HPLC-ECD)法测定各组大鼠前额皮质(mPFC)、伏隔核(Nac)和中脑腹侧被盖区(VTA)的DA与3,4-二羟基苯乙酸(DOPAC)含量.结果与对照组mPFC、Nac、VTA区的DA含量[(0.36±0.15)、(3.47±0.52)、(1.35±0.31)μg/g]相比,心理应激组相应区的DA含量[(0.08±0.05)、(1.88±0.27)、(1.01±0.26)μg/g]降低(P＜0.05),DOPAC/DA升高(P＜0.01).与心理应激组相比,Tyr 500 mg/kg干预组和1000 mg/kg干预组mPFC、Nac、VTA区的DA含量增加(P＜0.05),mPFC、VTA区的DOPAC/DA降低(P＜0.05).结论心理应激导致大鼠mPFC、Nac、VTA区的DA含量下降和DA更新率升高；Tyr干预能提高心理应激大鼠VTA-Nac-mPFC脑区的DA含量,降低DA更新率.
목적 탐토락안산(Tyr)간예대심리응격대서중추다파알(DA)함량급기경신솔적영향.방법 40지SD대서안수자표법수궤분위5조:대조조、심리응격조화3개제량Tyr간예조(분별경사료매천급여Tyr 250、500、1000 mg/kg),매조8지.건립대서Communication Box심리응격모형,채용고효액상색보-전화학(HPLC-ECD)법측정각조대서전액피질(mPFC)、복격핵(Nac)화중뇌복측피개구(VTA)적DA여3,4-이간기분을산(DOPAC)함량.결과 여대조조mPFC、Nac、VTA구적DA함량[(0.36±0.15)、(3.47±0.52)、(1.35±0.31)μg/g]상비,심리응격조상응구적DA함량[(0.08±0.05)、(1.88±0.27)、(1.01±0.26)μg/g]강저(P＜0.05),DOPAC/DA승고(P＜0.01).여심리응격조상비,Tyr 500 mg/kg간예조화1000 mg/kg간예조mPFC、Nac、VTA구적DA함량증가(P＜0.05),mPFC、VTA구적DOPAC/DA강저(P＜0.05).결론 심리응격도치대서mPFC、Nac、VTA구적DA함량하강화DA경신솔승고；Tyr간예능제고심리응격대서VTA-Nac-mPFC뇌구적DA함량,강저DA경신솔.
Objective To investigate the effects of central tyrosine on the dopamine content and its turnover rate in rats with psychological stress.Methods Forty SD rats were randomly divided into 5 groups:the control group,the psychological stress group,and 3-dose tyrosine intervention groups( fed with tyrosine with doses of 250 mg/kg,500 mg/kg and 1000 mg/kg respectively),each consisting of 8 rats.The psychological stress model was developed with the Communication Box.Contents of dopamine (DA) and dihydroxy-phenyl acetic acid (DOPAC) in the mesoprefrontal cortex (mPFC),nucleus accumbens (Nac) and ventral tegmental area(VTA) were determined by HPLC-ECD.Results The content of DA declined and that of DOPAC/DA increased significantly in mPFC,Nac and VTA area in the animals of psychological stress treatment group [ (0.08 ± 0.05 )、( 1.88 ± 0.27 )、( 1.01 ± 0.26 ) μg/g ]( P ＜ 0.05 ),when a comparison was made with the animals of the control group[(0.36 ± 0.15 )、(3.47 ± 0.52 )、( 1.35 ± 0.31 ) μg/g ].As compared with those of the psychological stress treatment group,the content of DA increased significantly in mPFC,Nac and VTA area (P ＜ 0.05 ) and DOPAC/DA declined significantly in mPFC and VTA area (P ＜ 0.05 ) in the 500 mg/kg and 1 000 mg/kg tyrosine intervention groups( P ＜0.05 ).Conclusions The content of DA could be depressed and the turnover rate of DA could be increased by psychological stress in the mPFC,Nac and VTA area,while tyrosine intervention could increase the content of DA and depress the turnover rate of DA in rats with psychological stress.