CAJ | 학술논문

目的观察c9,t11-CLA及t10,c12-CLA干预后,大鼠骨髓细胞过氧化物酶体增殖物激活受体2(PPARγ2)及核因子(NF)-κB活化受体配体(RANKL)、碱性磷酸酶(ALP)、骨保护素(OPG)、NF-κB活化受体(RANK)、抗酒石酸酸性磷酸酶(TRAP) mRNA表达的变化,探讨其对骨代谢的影响.方法体外培养大鼠骨髓细胞,分别加入c9,t11-CLA及t10,c12-CLA干预24h,逆转录-聚合酶链反应(RT-PCR)检测PPARγ2、RANKL、ALP、OPG、RANK、TRAP mRNA表达水平,比较其对骨髓细胞上述基因表达的影响.结果 (1)c9,t11-CLA呈剂量依赖性下调RANK、TRAP mRNA表达,组间比较差异有统计学意义(P＜0.05,P＜0.01),而其对RANKL、ALP、OPG及PPARγ2 mRNA 表达的影响不明显.(2) t10,c12-CLA呈剂量依赖性上调RANKL、OPG mRNA的表达,同时下调 RANK、TRAP及PPARγ2 mRNA的表达,组间比较差异有统计学意义(P＜0.05,P＜0.01),但对ALP mRNA的表达无明显影响.结论 c9,t11-CLA可能通过抑制破骨细胞标记物基因表达阻断骨髓细胞向破骨细胞分化,而t10,c12-CLA在抑制破骨细胞标记物基因表达的同时也可促进成骨细胞标记物基因表达,两者均有利于骨形成.
목적 관찰c9,t11-CLA급t10,c12-CLA간예후,대서골수세포과양화물매체증식물격활수체2(PPARγ2)급핵인자(NF)-κB활화수체배체(RANKL)、감성린산매(ALP)、골보호소(OPG)、NF-κB활화수체(RANK)、항주석산산성린산매(TRAP) mRNA표체적변화,탐토기대골대사적영향.방법 체외배양대서골수세포,분별가입c9,t11-CLA급t10,c12-CLA간예24h,역전록-취합매련반응(RT-PCR)검측PPARγ2、RANKL、ALP、OPG、RANK、TRAP mRNA표체수평,비교기대골수세포상술기인표체적영향.결과 (1)c9,t11-CLA정제량의뢰성하조RANK、TRAP mRNA표체,조간비교차이유통계학의의(P＜0.05,P＜0.01),이기대RANKL、ALP、OPG급PPARγ2 mRNA 표체적영향불명현.(2) t10,c12-CLA정제량의뢰성상조RANKL、OPG mRNA적표체,동시하조 RANK、TRAP급PPARγ2 mRNA적표체,조간비교차이유통계학의의(P＜0.05,P＜0.01),단대ALP mRNA적표체무명현영향.결론 c9,t11-CLA가능통과억제파골세포표기물기인표체조단골수세포향파골세포분화,이t10,c12-CLA재억제파골세포표기물기인표체적동시야가촉진성골세포표기물기인표체,량자균유리우골형성.
Objective To observe the effect of conjugated linoleic acid (c9,t11-CLA and t10,c12-CLA) on the mRNA expression of peroxisome proliferator activated receptorγ2 (PPARγ2),receptor activator of NF-κB ligand ( RANKL),alkaline phosphatase ( ALP),osteoprotegerin ( OPG),receptor activator of NF-κB (RANK) and tartrate-resistant acid phosphatase (TRAP) of bone marrow cells,and study the effect of c9,t11-CLA and t10,c12-CLA on bone metabolism.Methods Bone marrow cells of Wistar rats were cultured in vitro and then the cells were cultured in DMEM with c9,t11-CLA or t10,c12-CLA at different concentrations (the final concentration was 0,12.5,25.0,50.0 μmol/L,respectively) for 24 h.Reverse transcription-polymerase chain reaction (RT-PCR) was performed to detect the mRNA expression of PPARγ2,RANKL,ALP,OPG,RANK and TRAP.Results Semi-quantitative RT-PCR analysis showed that c9,t11-CLA down-regulated the mRNA expression of RANK and TRAP in a dose-dependent manner,and statistically significant difference was found in interclass comparison (P ＜ 0.05,P ＜ 0.01 ),while it had no effect on the mRNA expression of RANKL,ALP,OPG and PPARγ2.The results also showed that t10,c12-CLA up-regulated the mRNA expression of RANKL and OPG in a dose-dependent manner and down-regnlated the mRNA expression of RANK,TRAP and PPARγ2 in a dose-dependent manner,statistically significant difference was found in interclass comparison ( P ＜ 0.05,P ＜ 0.01 ),while it had no effect on the mRNA expression of ALP.Conclusion Our findings suggest that c9,t11-CLA and t10,c12-CLA can suppress the expression of osteoclastogenesis genes and t10,c12-CLA can also promote the expression of osteogenic genes,which suggests a possible benecial effect on bone formation.