CAJ | 학술논문

目的观察脆性X综合征(FXS)模型小鼠不同发育时期脑组织中microRNA-134(miR-134)的表达,明确miR-134的表达特点及脆性X智力低下蛋白(FMRP)缺失是否导致miR-134转录的改变. 方法应用荧光实时定量PCR检测FVB近交系雄性0 d、4、6周(W)龄FMR1基因敲除型(KO)(KO0d、KO4w、KO6w)和同龄野生型(WT)(WT0d、WT4w、WT6w)小鼠脑组织中miR-134的表达(n=5). 结果同龄KO与WT小鼠miR-134的转录表达量差异无统计学意义(P＞0.05);KO6w小鼠脑组织miR-134的转录表达量低于KO0d和KO2w小鼠,WT6w小鼠脑组织miR-134的转录表达量也低于WT0d和WT2w小鼠,差异均有统计学意义(P＜0.05). 结论 FMRp的缺失并未影响miR-134的转录;miR-134的转录表达量在神经系统发育期保持着较高水平,至成年期则下降,提示其在调控神经系统发育中可能起着重要作用.
목적 관찰취성X종합정(FXS)모형소서불동발육시기뇌조직중microRNA-134(miR-134)적표체,명학miR-134적표체특점급취성X지력저하단백(FMRP)결실시부도치miR-134전록적개변. 방법 응용형광실시정량PCR검측FVB근교계웅성0 d、4、6주(W)령FMR1기인고제형(KO)(KO0d、KO4w、KO6w)화동령야생형(WT)(WT0d、WT4w、WT6w)소서뇌조직중miR-134적표체(n=5). 결과동령KO여WT소서miR-134적전록표체량차이무통계학의의(P＞0.05);KO6w소서뇌조직miR-134적전록표체량저우KO0d화KO2w소서,WT6w소서뇌조직miR-134적전록표체량야저우WT0d화WT2w소서,차이균유통계학의의(P＜0.05). 결론 FMRp적결실병미영향miR-134적전록;miR-134적전록표체량재신경계통발육기보지착교고수평,지성년기칙하강,제시기재조공신경계통발육중가능기착중요작용.
Objective To observe the expression ofmicroRNA-134 (miR-134) in the mouse brain tissue with FMR1 gene knockout during the different development periods and its expression characteristic, and explore whether the deficiency of fragile X mental retardation protein (FMRP) can induce the changes of miR-134 transcription. Methods FVB strain male mice, including FMR1 gene knockout (KO, n=15) and their wild type (WT, n=15) counterparts were chosen in the experiment. The expressions of miR-134 in the brain tissues of these KO mice that were 0 d, 4 and 6 w old and the age-matched WT mice were detected by qRT-PCR. Results The transcriptional level of miR-134 in the brain tissue of KO mice had no significant difference as compared with that of age-matched WT mice (P＞0.05). The transcriptional levels of miR-134 in 6-w-old KO and WT mice were significantly decreased as compared with the newbom and 4-w-old same genotype mice (P＜0.05). Conclusion The absence of FMRP does not influence the transcription of miR-134 and the transcriptional level of miR-134 in the brain tissues maintains a high level during the developmental stage of the nervous system and gradually decreases to a low level after grow-up, demonstrating its important role in regulating the development of nervous system.