CAJ | 학술논문

目的探讨卵巢癌耐药基因表达与体外药敏试验及临床生物学行为的关系.方法采用流式细胞术和MTT法对60例卵巢癌患者分别检测组织中的COX-2、CX43、P-gp基因表达和对常用化疗药物的敏感性,并对其临床近期疗效关系进行分析.结果 COX-2表达在顺铂(DDP)耐药组高于DDP敏感组,差异有统计学意义(P=0.01).CX43表达在DDP耐药组低于DDP敏感组,差异有统计学意义(P=0.005).P-gp表达在对依托泊苷(VP16)耐药组高于VP16敏感组,差异有统计学意义(P=0.009).CX43表达在对紫杉醇(Taxol)耐药组低于Taxol敏感组.差异有统计学意义(P=0.001).COX-2高表达、CX43低表达、P-gp高表达的癌组织对化疗药物的敏感性低于COX-2低表达、CX43高表达、P-gP低表达者.5-Fu、CBP、VCR、PYM、EPI、GEM的敏感性与COX-2、CX43、P-gp表达高低无显著相关性.结论卵巢癌组织中COX-2与P-gp高表达、CX43低表达与部分化疗药物的体外药敏试验敏感性有关.耐药基因相关蛋白COX-2、P-gp、CX43的检测,结合肿瘤药敏试验结果选择敏感化疗方案能提高化疗疗效.从而使卵巢癌治疗个体化.
목적 탐토란소암내약기인표체여체외약민시험급림상생물학행위적관계.방법 채용류식세포술화MTT법대60례란소암환자분별검측조직중적COX-2、CX43、P-gp기인표체화대상용화료약물적민감성,병대기림상근기료효관계진행분석.결과 COX-2표체재순박(DDP)내약조고우DDP민감조,차이유통계학의의(P=0.01).CX43표체재DDP내약조저우DDP민감조,차이유통계학의의(P=0.005).P-gp표체재대의탁박감(VP16)내약조고우VP16민감조,차이유통계학의의(P=0.009).CX43표체재대자삼순(Taxol)내약조저우Taxol민감조.차이유통계학의의(P=0.001).COX-2고표체、CX43저표체、P-gp고표체적암조직대화료약물적민감성저우COX-2저표체、CX43고표체、P-gP저표체자.5-Fu、CBP、VCR、PYM、EPI、GEM적민감성여COX-2、CX43、P-gp표체고저무현저상관성.결론 란소암조직중COX-2여P-gp고표체、CX43저표체여부분화료약물적체외약민시험민감성유관.내약기인상관단백COX-2、P-gp、CX43적검측,결합종류약민시험결과선택민감화료방안능제고화료료효.종이사란소암치료개체화.
Objective To study the relation between the expression of drug-resistance gene and in vitro drug sensitivity test in ovarian carcinoma and clinical biological behavior.Methods In 60 primarily treated patients with epithelial ovarian carcinoma,the expression of COX-2,Connexin43(CX43)and P-gp was detected using flow cytometry.Sensitivity of cells of short term in vitro culture to anticancer drugs was also examined by the game techniques.Remits The expression level of COX-2 was higher in tissue with DDP-resistance patients than those with sensitivity patients but the expression level of CX43 was lower in tissue with DDP-resistance and Taxol-resistanee patients than those with sensitivity patients.The expression level of P-gp was higher in tissue with VP16-resistance patients than those with sensitivity patients.The sensitivity of DDP,Taxol and VP16 was lower in tissue with higher expression of COX-2 and P-gp than those with lower expression of COX-2 and P-gp.The sensitivity of DDP and Taxol was higher in tissue with higher expression of CX43 than those with lower expression of CX43.Conclusion The in vitro sensitivity of some of the drugs is effected by tissues with expression of COX-2,P-gp and CX43.It is not only useful but also individualized treatment for application of sensitive drug test and detecting the expression of COX-2,P-gP and CX43 to increase the response rate of chemotherapy in patients with epithelial ovarian carcinoma.