中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2009年
19期
1491-1494
,共4页
董建德%张建%谷涌泉%李春民%王春仁%陈兵%李建新%吴英锋%张淑文%孟艳%汪忠镐
董建德%張建%穀湧泉%李春民%王春仁%陳兵%李建新%吳英鋒%張淑文%孟豔%汪忠鎬
동건덕%장건%곡용천%리춘민%왕춘인%진병%리건신%오영봉%장숙문%맹염%왕충호
组织工程%间质干细胞%细胞外基质%生物反应器
組織工程%間質榦細胞%細胞外基質%生物反應器
조직공정%간질간세포%세포외기질%생물반응기
Tissue engineering%Mesenchymal stem cells%Extracellular matrix%Bioreactors
目的 探讨利用异种脱细胞血管基质和间充质干细胞体外构建小口径血管移植物的方法.方法 采用去垢剂和胰蛋白酶去除猪髂动脉血管壁的细胞成分,对脱细胞基质进行组织学、力学检测及孔隙率评估.分离培养犬骨髓问充质干细胞,种植到脱细胞基质上,并进一步在搏动性生物反应器内培养,采用HE染色和扫描电镜对构建的组织工程血管进行检测.结果 脱细胞处理后,猪髂动脉的细胞成分完全去除,细胞外基质保存完好,力学强度轻度下降;脱细胞基质的孔隙率为94.9%.间充质干细胞能够种植到脱细胞基质上,在剪切力的作用下细胞基本融合,高度伸长并且其排列与流体的方向一致.结论 小口径血管移植物可以通过将间充质干细胞种植到异种脱细胞血管基质并在搏动性生物反应器内培养的方法进行构建.
目的 探討利用異種脫細胞血管基質和間充質榦細胞體外構建小口徑血管移植物的方法.方法 採用去垢劑和胰蛋白酶去除豬髂動脈血管壁的細胞成分,對脫細胞基質進行組織學、力學檢測及孔隙率評估.分離培養犬骨髓問充質榦細胞,種植到脫細胞基質上,併進一步在搏動性生物反應器內培養,採用HE染色和掃描電鏡對構建的組織工程血管進行檢測.結果 脫細胞處理後,豬髂動脈的細胞成分完全去除,細胞外基質保存完好,力學彊度輕度下降;脫細胞基質的孔隙率為94.9%.間充質榦細胞能夠種植到脫細胞基質上,在剪切力的作用下細胞基本融閤,高度伸長併且其排列與流體的方嚮一緻.結論 小口徑血管移植物可以通過將間充質榦細胞種植到異種脫細胞血管基質併在搏動性生物反應器內培養的方法進行構建.
목적 탐토이용이충탈세포혈관기질화간충질간세포체외구건소구경혈관이식물적방법.방법 채용거구제화이단백매거제저가동맥혈관벽적세포성분,대탈세포기질진행조직학、역학검측급공극솔평고.분리배양견골수문충질간세포,충식도탈세포기질상,병진일보재박동성생물반응기내배양,채용HE염색화소묘전경대구건적조직공정혈관진행검측.결과 탈세포처리후,저가동맥적세포성분완전거제,세포외기질보존완호,역학강도경도하강;탈세포기질적공극솔위94.9%.간충질간세포능구충식도탈세포기질상,재전절력적작용하세포기본융합,고도신장병차기배렬여류체적방향일치.결론 소구경혈관이식물가이통과장간충질간세포충식도이충탈세포혈관기질병재박동성생물반응기내배양적방법진행구건.
Objective To investigate the method of constructing small-diameter vascular grafts from xenogenic decellularized arterial matrices and mesenchymal stem cells (MSCs). Methods Porcine iliac arteries were decellularized by detergent and trypsin treatment. Histology, mechanical strength and porosity experiments were performed to evaluate the properties of decellularized matrices. MSCs were isolated from bone marrow of dogs and expanded ex vivo. Decellularized matrices were seeded with MSCs and further cultured in a pulsatile biorcactor. Morphological features of the tissue engineered grafts were assayed by HE staining and scanning electron microscopy. Results After cell extraction, absence of cellular components and preservation of extracellular matrix were verified. Mechanical strength of decellularized matrices was slightly reduced compared with native arteries. Porosity of decellularized matrices was 94.9%. Decellularized matrices were successfully seeded with MSCs, which grew to a near-confluent monolayer under flow conditions and MSCs were highly elongated and oriented to the flow direction. Conclusion Small-diameter vascular grafts can be constructed by seeding MSCs onto xenogenic decellularized arterial matrices and culturing in a pulsatile bioreactor.
