CAJ | 학술논문

高等植物中甜菜碱由胆碱经两步氧化反应合成,其中由胆碱单加氧酶(CMO)催化第一步氧化反应,甜菜碱醛脱氢酶(BADH)催化第二步氧化反应.采用生物信息学技术和RT-PCR的方法从水稻幼苗组织中分离得到了水稻CMO基因的cDNA克隆,将其命名为OsCMO.该基因含有10个外显子和9个内含子,其开放阅读框编码一条长为410个氨基酸残基的多肽,与拟南芥、山菠菜、苋、碱蓬和甜菜等植物的胆碱单加氧酶的氨基酸序列的一致性50%～62%.mRNA分析表明OsCMO基因在水稻幼苗组织中的表达受高盐、低温和干旱等胁迫的上调诱导表达,表明该基因可能在抵御非生物胁迫中发挥重要作用.
고등식물중첨채감유담감경량보양화반응합성,기중유담감단가양매(CMO)최화제일보양화반응,첨채감철탈경매(BADH)최화제이보양화반응.채용생물신식학기술화RT-PCR적방법종수도유묘조직중분리득도료수도CMO기인적cDNA극륭,장기명명위OsCMO.해기인함유10개외현자화9개내함자,기개방열독광편마일조장위410개안기산잔기적다태,여의남개、산파채、현、감봉화첨채등식물적담감단가양매적안기산서렬적일치성50%～62%.mRNA분석표명OsCMO기인재수도유묘조직중적표체수고염、저온화간한등협박적상조유도표체,표명해기인가능재저어비생물협박중발휘중요작용.
In plants, the osmoprotectant glycine betaine (GB) is synthesized via the pathway that choline monooxygenase (CMO) oxidizes choline to betaine aldehyde, which is subsequently oxidized to GB by betaine aldehyde dehydrogenase (BADH). Although rice is considered as a typical non-glycinebetaine accumulating species, a cDNA encoding putative choline monooxygenase (CMO) was isolated from rice by RT-PCR and designated as OsCMO. The OsCMO contains 10 exons and 9 introns and encodes a protein with 410 amino acids,which shows 62%, 51%, 51%, 51% and 50% identities in amino acids to CMOs from Arabidopsis thaliana,Atriplex hortensis, Amaranthus tricolor, Suaeda liaotungensis and Beta vulgaris respectively. Semi-quantitative RT-PCR assay revealed OsCMO was significantly up-regulated in the rice seedlings under salt, cold and drought stresses. It suggests that OsCMO might play an important role in rice responses to abiotic stresses.