CAJ | 학술논문

背景:以有机大分子作为矿化模版进行骨组织修复材料的仿生构建,是目前骨修复材料的研究热点.而将琼脂应用于骨修复材料的报道较少.目的:仿生合成一种由琼脂和羟基磷灰石组成的新型纳米复合骨组织修复材料,评价其理化性能和细胞相容性.方法:①将一定量的非纳米羟基磷灰石的盐酸溶液,加入一定量的琼脂溶胶中,调整反应体系的pH值至7～8,然后将反应形成的复合物冷冻干燥后即得琼脂-羟基磷灰石复合材料.②将第3代SD大鼠骨髓基质细胞与琼脂-羟基磷灰石复合材料共培养,于培养1,3,5 d时观察细胞生长情况.结果与结论:X射线衍射仪,傅里叶变换红外光谱仪,热分析仪,透射电镜和扫描电镜对材料进行表征分析,显示琼脂良好控制了磷灰石晶体的生长,纳米的磷灰石晶体均匀分布在琼脂纤维中,琼脂-羟基磷灰石复合物具有多孔结构.共培养3,5 d时骨髓基质细胞在复合物中生长良好,并有较明显的细胞骨架形成.提示琼脂-羟基磷灰石复合物具有良好的理化性质和细胞相容性.
배경:이유궤대분자작위광화모판진행골조직수복재료적방생구건,시목전골수복재료적연구열점.이장경지응용우골수복재료적보도교소.목적:방생합성일충유경지화간기린회석조성적신형납미복합골조직수복재료,평개기이화성능화세포상용성.방법:①장일정량적비납미간기린회석적염산용액,가입일정량적경지용효중,조정반응체계적pH치지7～8,연후장반응형성적복합물냉동간조후즉득경지-간기린회석복합재료.②장제3대SD대서골수기질세포여경지-간기린회석복합재료공배양,우배양1,3,5 d시관찰세포생장정황.결과여결론:X사선연사의,부리협변환홍외광보의,열분석의,투사전경화소묘전경대재료진행표정분석,현시경지량호공제료린회석정체적생장,납미적린회석정체균균분포재경지섬유중,경지-간기린회석복합물구유다공결구.공배양3,5 d시골수기질세포재복합물중생장량호,병유교명현적세포골가형성.제시경지-간기린회석복합물구유량호적이화성질화세포상용성.
BACKGROUND: Studies regarding synthesizing composite bone repair materials with organic macromolecules as mineralizers template are the hot research spot. However, reports concerning using agar as bone repair materials are few. OBJECTIVE: To synthesize a novel agar-hydroxyapatite composite material, and to evaluate its physical and chemical properties as well as cytocompatibility.METHODS: ①A certain amount of the non-nano-hydroxyapatite in hydrochloric acid solution was added into a certain amount of agar sol, and the reaction system was adjusted with PH value of 7-8. And then the precipitate was lyophilized to obtain the composite of agar-hydroxyapatite.②The third generation of rat bone marrow stromal cell (BMSC) was co-cultured with agar-hydroxyapatite. And the growth of cells was observed at days 1, 3 and 5 after culture. RESULTS AND CONCLUSION: The results of X-ray diffraction patterns, Fourier transform infrared spectrometer (FTIR), thermal analyzer, transmission electron microscope, and scanning electron microscope showed that agar could manipulate the growth of hydroxyapatite, and hydroxyapatite nanocrystals was equably formed on the agar-fibers with high porosity. The BMSC grew well in the composite and form a clear cytoskeletal at days 3 and 5 after culture. The results reveal that agar-hydroxyapatite composite has good physical and chemical properties and cytocompatibility.