CAJ | 학술논문

桑树内生拮抗细菌枯草芽孢杆菌(Bacillus subtilis) L144对多种植物病原菌具有较强的抑制作用.为进一步探讨该内生菌的开发应用价值,研究了该内生菌对植物生长及营养代谢的影响.试验结果表明:适当浓度的L144菌株培养液能明显提高玉米和桑树种子的发芽势、发芽率和胚根长,增加玉米和桑树幼苗的株高及植株鲜质量和干质量,提高植株体内叶绿素、可溶性蛋白和可溶性糖的含量,且以菌株培养液10倍稀释液的促生作用最为显著;该菌株的菌体悬浮液及胞外分泌物对玉米幼苗也表现出不同程度的促生作用;该菌株还具有分泌植物生长激素、溶解无机磷和有机磷的性能,有可能是其促进植株生长的主要作用机制.试验结果提示,L144菌株的培养液及菌体和胞外分泌物对植物有较明显的促生作用.
상수내생길항세균고초아포간균(Bacillus subtilis) L144대다충식물병원균구유교강적억제작용.위진일보탐토해내생균적개발응용개치,연구료해내생균대식물생장급영양대사적영향.시험결과표명:괄당농도적L144균주배양액능명현제고옥미화상수충자적발아세、발아솔화배근장,증가옥미화상수유묘적주고급식주선질량화간질량,제고식주체내협록소、가용성단백화가용성당적함량,차이균주배양액10배희석액적촉생작용최위현저;해균주적균체현부액급포외분비물대옥미유묘야표현출불동정도적촉생작용;해균주환구유분비식물생장격소、용해무궤린화유궤린적성능,유가능시기촉진식주생장적주요작용궤제.시험결과제시,L144균주적배양액급균체화포외분비물대식물유교명현적촉생작용.
Mulberry antagonistic endophytic bacterium Bacillus subtilis L144 has strong inhibitory effect on various plant patho-gens.In order to further explore the development and application value of this bacterium so as to provide new resources for plant microbioecologics,its effects on growth promotion and nutrition metabolism of plants were investigated.The results showed that the culture solution of bacterial strain L144 at certain concentrations could heighten the germinability,germination rate and radicular length of maize and mulberry seeds obviously,increase seedling height and plantlet fresh weight and dry weight,and improve the contents of chlorophyll,soluble protein and soluble carbohydrate in plantlets.The growth promo-tion effect of 10 time dilution of the culture solution was the most significant.Furthermore,bacterial suspension solution and secreted substances of this bacterium had certain effects on promoting growth of maize seedlings.This bacterial strain could also secrete plant growth hormone and dissolve inorganic and organic phosphorous compounds,possiblyaccounting for its promotion effect on plant growth.The obtained results demonstrate that the culture solution,the bacterial suspension and secreted subst:ances of strain L144 have obvious effect on promoting plant growth.