CAJ | 학술논문

目的探讨高糖对内皮细胞转分化的影响及其与血管紧张素Ⅱ(ATⅡ)的关系.方法将人主动脉内皮细胞分成正常浓度葡萄糖(NG)组、高糖(HG)组和厄贝沙坦干预(HG+Irb)组.放射免疫法检测细胞上清液中ATⅡ的浓度.共聚焦显微镜观察CD31和成纤维细胞特异蛋白1(FSP1)的双染色结果.Western blot检测FSP1蛋白水平的表达.结果与NG组比,高糖刺激的内皮细胞导致ATⅡ和FSP1表达增加(P<0.05),呈浓度和时间依赖性.共聚焦显微镜可见CD31和FSP1表达重叠,且一些细胞获得纺锤样的改变并失去CD31染色.厄贝沙坦可抑制高糖引起的上述改变(P<0.05).结论高糖可能通过ATⅡ介导的内皮细胞转分化导致内皮细胞损伤,而厄贝沙坦抑制内皮细胞转分化.
목적 탐토고당대내피세포전분화적영향급기여혈관긴장소Ⅱ(ATⅡ)적관계.방법 장인주동맥내피세포분성정상농도포도당(NG)조、고당(HG)조화액패사탄간예(HG+Irb)조.방사면역법검측세포상청액중ATⅡ적농도.공취초현미경관찰CD31화성섬유세포특이단백1(FSP1)적쌍염색결과.Western blot검측FSP1단백수평적표체.결과 여NG조비,고당자격적내피세포도치ATⅡ화FSP1표체증가(P<0.05),정농도화시간의뢰성.공취초현미경가견CD31화FSP1표체중첩,차일사세포획득방추양적개변병실거CD31염색.액패사탄가억제고당인기적상술개변(P<0.05).결론 고당가능통과ATⅡ개도적내피세포전분화도치내피세포손상,이액패사탄억제내피세포전분화.
Objective The aim was to investigate the influence of high glucose on endothelial to mesenchymal transition (EndMT) and its relevance with the activation of angiotensin Ⅱ. Methods Human aortic endothelial cells (HAEC) were divided into three groups: normal glucose group (NG), high glucose group (HG) and the Irbesartan (1μmol/L) treated group. The concentration of angiotensin Ⅱ in the HAEC supernatant was detected by radioimmunoassay. Immunofluorescence staining was performed to detect the co-expression of CD31 and fibroblast specific protein1 (FSP1). Results HG led to a significant increase of expression of angiotensin Ⅱ and FSP1 protein in HAEC in dose and time dependent manner. Double staining of HAEC showed co-localization of CD31 and FSP1. These changes were inhibited by treatment with irbesartan (P<0.05). Conclusion These findings suggest a novel mechanism on the high glucose HG induced endothelial damage through angiotensin II mediated EndMT, which is inhibited by irbesartan.