CAJ | 학술논문

用差速离心法和改进的碱裂解法分离纯化遵义小钝吻鸢毛线粒体DNA，紫外检测其OD260/OD280在1．78-1．85，并计算出该鱼肝组织线粒体DNA的含量为0．613μg/g。纯化样品经紫外分析仪在200-290nm波长范围内扫描，呈现典型的DNA吸收峰，其最大吸收峰在259～260nm。以hDNA／HindIII的完全酶切片段作为相对分子量标准，利用电泳迁移率与分子量的对数的线性关系，由已知片段大小推算出遵义小钝吻鸵mtDNA分子大小为（15．44±0．16）kb。
용차속리심법화개진적감렬해법분리순화준의소둔문연모선립체DNA，자외검측기OD260/OD280재1．78-1．85，병계산출해어간조직선립체DNA적함량위0．613μg/g。순화양품경자외분석의재200-290nm파장범위내소묘，정현전형적DNA흡수봉，기최대흡수봉재259～260nm。이hDNA／HindIII적완전매절편단작위상대분자량표준，이용전영천이솔여분자량적대수적선성관계，유이지편단대소추산출준의소둔문타mtDNA분자대소위（15．44±0．16）kb。
The mitochondrial DNA（mtDNA）from liver ofLeioxassis~ticrocrassirostris ZunyiensisXiao sp.nov.was isolated and purified by means of differential centrifugation and the improved alkaline lysisoThe OD260/OD280 was 1.78-1.85 by UV spectrophotometer,and the quality of mtDNA obtained was 0.613 μg/goUnder the wavelength scan of 200-290 nm by UV solution ,the Purification of DNA of the sample presented characteristic DNA absorption peaks and the maximum one in 259-260 nmoUsekDNA/Hind m restriction fragments as a relatively molecular weight standard, according to the electrephoretic migration rate was linearly related to the molecular weight, the mtDNA size was （15.44±0.16） kb in the fish .