CAJ | 학술논문

目的探讨射频消融(RFA)治疗大鼠肝肿瘤前后血清TGF-β1、外周血IL-10的变化及其意义.方法采用瘤块包埋法制作SD大鼠Walker-256肝肿瘤模型30只,随机分为RFA 1周组、RFA 2周组和对照组,每组10只,前两组分别在RFA后1周、RFA后2周处死动物,对照组不做RFA即处死动物并取外周血,采用Ficoll密度梯度离心法分离外周血单个核细胞,应用ELISA法检测血清TGF-β1,应用FCM检测其IL-10水平.结果 ①对照组大鼠血清TGF-β1水平(6.61±0.12)μg/L.RFA后1周为(5.63±0.46)μg/L,RFA后2周为(5.53±0.56)μg/L,RFA后1周组及RFA后2周组与对照组比较差异均有统计学意义(P＜0.05).②对照组大鼠外周血IL-10表达水平95.92±2.31,RFA后1周为89.71±5.44,RFA后2周为87.67±11.11,RFA后1周组与对照组比较差异有统计学意义(P＜0.05).结论 RFA通过对肿瘤组织的原位灭活在一定程度上解除肿瘤组织所释放的TGF-β1、IL-10对荷瘤宿主的免疫抑制状态,促进荷瘤宿主体内树突状细胞的分化成熟,提高机体抗原提呈能力.
목적 탐토사빈소융(RFA)치료대서간종류전후혈청TGF-β1、외주혈IL-10적변화급기의의.방법 채용류괴포매법제작SD대서Walker-256간종류모형30지,수궤분위RFA 1주조、RFA 2주조화대조조,매조10지,전량조분별재RFA후1주、RFA후2주처사동물,대조조불주RFA즉처사동물병취외주혈,채용Ficoll밀도제도리심법분리외주혈단개핵세포,응용ELISA법검측혈청TGF-β1,응용FCM검측기IL-10수평.결과 ①대조조대서혈청TGF-β1수평(6.61±0.12)μg/L.RFA후1주위(5.63±0.46)μg/L,RFA후2주위(5.53±0.56)μg/L,RFA후1주조급RFA후2주조여대조조비교차이균유통계학의의(P＜0.05).②대조조대서외주혈IL-10표체수평95.92±2.31,RFA후1주위89.71±5.44,RFA후2주위87.67±11.11,RFA후1주조여대조조비교차이유통계학의의(P＜0.05).결론 RFA통과대종류조직적원위멸활재일정정도상해제종류조직소석방적TGF-β1、IL-10대하류숙주적면역억제상태,촉진하류숙주체내수돌상세포적분화성숙,제고궤체항원제정능력.
Objective To discuss the change of serum TGF-β1 and IL-10 in peripheral blood of rats with liver tumor treated by RFA.Methods Thirty experimental liver tumor model of SD rats were prepared by implantation of tumor particles.These rats were randomly divided equally into three groups including 1 week after RFA,2 week after RFA and control group.Peripheral blood of control group,group 1 week after RFA and group 2 week after RFA was taken out respectively without RFA,1 week and 2 week after RFA.The mononuclear cells of peripheral blood were separated by Ficoll density gradient centrifugation.The expression level of IL-10 in peripheral blood was analyzed with flowcytometry.Serum TGF-β1 were dectected by ELISA.Results The serum expression level of TGF-β1 of control group was(6.61±0.12)μg/L,and that of group 1 week and 2 week after RFA were respectively(5.63±0.46)μg/L and(5.53±0.56)μg/L.There was statistical significance for the difference between control group and group 1 week or group 2 week after RFA.The serum expression level of IL-10 of control gorup was 95.92±2.31,and that of group 1 week and 2 week after RFA were respectively 89.71±5.44 and 87.67±11.11.There was statistical significance for the difference between control group and group 1 week after RFA.Conclusions RFA can destroy the tumor tissues in situ and relief immune suppression by IL-10,TGF-β1 secreted by tumor tissue.RFA can improve differentiation and maturation of dendritic cell in local area of tumor and promate ability of antigen-presentation of body.