CAJ | 학술논문

目的研究糖尿病大鼠心肌连接蛋白(Connexin43)的变化.方法使用链脲佐菌素(STZ)建立糖尿病大鼠模型.经右颈总动脉插管采用电生理记录仪及超声心动图检测心功能的变化,应用免疫组化及图像分析技术观察心肌细胞CX43的改变.结果糖尿病组大鼠心肌细胞Cx43表达明显减少,以24周组更明显(P均<0.01),12周及24周组的最大舒张期压力变化速率(-dp/dtmax)、最大收缩期压力变化速率(+dp/dtmax)均显著下降(P<0.01),左室射血分数(LVEF)亦明显降低(12周P<0.05;24周P<0.01),Cx43与-dp/dtmax(r=0.564,P<0.01)、+dp/dtmax、LVEF(r=0.426,0.414,P<0.05)呈正相关.结论糖尿病大鼠心肌Cx43表达明显减少,心功能减低,两者具有明显相关性.
목적 연구당뇨병대서심기련접단백(Connexin43)적변화.방법 사용련뇨좌균소(STZ)건립당뇨병대서모형.경우경총동맥삽관채용전생리기록의급초성심동도검측심공능적변화,응용면역조화급도상분석기술관찰심기세포CX43적개변.결과 당뇨병조대서심기세포Cx43표체명현감소,이24주조경명현(P균<0.01),12주급24주조적최대서장기압력변화속솔(-dp/dtmax)、최대수축기압력변화속솔(+dp/dtmax)균현저하강(P<0.01),좌실사혈분수(LVEF)역명현강저(12주P<0.05;24주P<0.01),Cx43여-dp/dtmax(r=0.564,P<0.01)、+dp/dtmax、LVEF(r=0.426,0.414,P<0.05)정정상관.결론 당뇨병대서심기Cx43표체명현감소,심공능감저,량자구유명현상관성.
Objective To study the change of the eonnxin43 expression in the heart of diabetic mellitus rats. Methods STZ-in-dueed diabetic mellitus in rats were developed. The expression of connexin43 in the heart was detected by immunohistochemistry and image analysis, and the heart function was observed by electrophysiolograph and echocardiogram. Results During the course of diabetic mellitus, the expression of connexin43 in the heart of diabetic rats decreased respectively in the 12th week and 24th week (P<0.01). The values of dp/dtmax and + dp/dtmax in group DM in the 12th week and 24th week were lower than that in normal control group respectively (P<0.01), and the LVEF were also decreased (12w P<0.05,24w P<0.01). The analysis of correlation showed that the expression of con-nexin43 in heart were positively correlated with heart function (P<0.05) . Conclusion The expression of Connexin43 evidently de-creased in the hearts of diabetic rats and it is positively correlated with the heart function.