CAJ | 학술논문

目的评价山莨菪碱对过度训练大鼠心肌细胞凋亡及炎性反应的影响.方法雄性Wistar大鼠24只,体重200～220 g,按随机数字表法分为3组(n=8):对照组(C组)、过度训练组(E组)和山莨菪碱组(A组).采用游泳至力竭法建立过度训练致大鼠心肌损伤模型.A组于过度训练前20 min腹腔注射山莨菪碱10 m∥ks.于过度训练后6 h时采集下腔静脉血后处死,取心肌组织,应用全自动生化仪检测血清CK-MB活性,免疫组化法检测心肌组织TNF-α含量及NF-κB活性,流式细胞仪检测心肌细胞凋亡率.结果与C组比较,E组和A组过度训练后6 h时血清CK-MB活性、心肌细胞凋亡率、心肌组织TNF-α含量及NF-κB活性升高(P＜0.05);与E组比较,A组过度训练后6 h时血清CK-MB活性、心肌细胞凋亡率、心肌组织TNF-α含量及NF-κB活性降低(P＜0.05).结论山莨菪碱可抑制过度训练大鼠心肌细胞凋亡,其机制与减轻心肌组织炎性反应有关.
목적 평개산랑탕감대과도훈련대서심기세포조망급염성반응적영향.방법 웅성Wistar대서24지,체중200～220 g,안수궤수자표법분위3조(n=8):대조조(C조)、과도훈련조(E조)화산랑탕감조(A조).채용유영지력갈법건립과도훈련치대서심기손상모형.A조우과도훈련전20 min복강주사산랑탕감10 m∥ks.우과도훈련후6 h시채집하강정맥혈후처사,취심기조직,응용전자동생화의검측혈청CK-MB활성,면역조화법검측심기조직TNF-α함량급NF-κB활성,류식세포의검측심기세포조망솔.결과 여C조비교,E조화A조과도훈련후6 h시혈청CK-MB활성、심기세포조망솔、심기조직TNF-α함량급NF-κB활성승고(P＜0.05);여E조비교,A조과도훈련후6 h시혈청CK-MB활성、심기세포조망솔、심기조직TNF-α함량급NF-κB활성강저(P＜0.05).결론 산랑탕감가억제과도훈련대서심기세포조망,기궤제여감경심기조직염성반응유관.
Objective To evaluate the effects of anisodamine on apoptosis in cardiomyocytes and inflammatory response in overtrained rats. Methods Twenty-four male Wistar rats, weighing 200-220 g, were randomly divided into 3 groups ( n = 8 each) : control group (group C) , overtraining group (group O) , anisodamine group (group A) . The model of overtraining-induced acute heart injury was established by exhausting swimming. Anisodamine 10 mg/kg was given intraperitoneally 20 min before overtraining in group A. Blood samples were taken at 6 h after overtraining for measurement of serum CK-MB activity. The rats were then sacrificed and myocardial tissues taken for determination of TNF-α content and NF-κB activity (by immunohistochemistry) . The apoptosis rate was detected by flow cytometry. Results The CK-MB activity, apoptosis rate, TNF-α content and NF-κB activity were significantly higher at 6 h after overtraining in groups O and A than in group C, while lower at 6 h after overtraining in group A than in group O ( P < 0.05) . Conclusion Anisodamine can inhibit apoptosis in cardiomyocytes by reducing inflammatory response in overtrained rats.