CAJ | 학술논문

目的动态观察种植体植入后非负荷期不同时间种植体周围核因子κB受体激活蛋白配体(receptor activator of NF-κB ligand,RANKL)与护骨因子的表达规律.方法建立Beagle犬牙种植动物模型,分别观测种植体植入后第3、7、15、30、60和90天种植体周围的骨改建情况.取种植体周围组织进行实时荧光定量聚合酶链反应,对硬组织切片进行形态学观察及定量组织学分析.结果骨改建的最活跃期为种植体植入后的第7天,种植体周围软组织及骨组织中的两种因子水平均随植入时间增加,第7天达高峰(护骨因子/RANKL mRNA 2.15±0.01),而后均逐渐降低.结论 RANKL与护骨因子在种植体周围组织中均有表达,其变化规律与骨组织改建过程一致；非负荷期种植体周围骨改建的活跃期大约在种植体植入后第7天.
목적 동태관찰충식체식입후비부하기불동시간충식체주위핵인자κB수체격활단백배체(receptor activator of NF-κB ligand,RANKL)여호골인자적표체규률.방법 건립Beagle견아충식동물모형,분별관측충식체식입후제3、7、15、30、60화90천충식체주위적골개건정황.취충식체주위조직진행실시형광정량취합매련반응,대경조직절편진행형태학관찰급정량조직학분석.결과 골개건적최활약기위충식체식입후적제7천,충식체주위연조직급골조직중적량충인자수평균수식입시간증가,제7천체고봉(호골인자/RANKL mRNA 2.15±0.01),이후균축점강저.결론 RANKL여호골인자재충식체주위조직중균유표체,기변화규률여골조직개건과정일치；비부하기충식체주위골개건적활약기대약재충식체식입후제7천.
Objective To observe the expression of receptor activator of NF-κB ligand (RANKL)and its decoy receptor osteoprotegerin (OPG) during unloading period of dental implants.Methods An animal model of dental implants was established in Beagle dogs.Bone remodeling was tested at 3,7,15,30,60 and 90 days after the placement of implants.RANKL and OPG mRNA expression were quantified by real-time PCR.Then mandibular bones were resected and some sections were observed.Results The most prominent period of bone remodeling occurred at 7 day after the placement of implants (OPG/RANKL mRNA,2.15 ±0.1).The expression of RANKL and OPG increased in a time-dependent manner in both soft and hard tissue.After 7 days they gradually decreased.Condusions Both OPG and RANKL were expressed in peri-implant tissues,and the changing tendency of RANKL and OPGmRNA was consistent with the change of bone remodeling.The active stage for bone remodelling in peri-implant tissues during unloading period is about 7 days after implantation.