CAJ | 학술논문

目的检测CIP2A mRNA在肝细胞癌中的表达,探讨其与临床病理因素和预后的关系.方法用半定量RT-PCR检测4株人肝癌细胞系、1株永生化肝细胞系以及120例肝细胞癌组织、癌旁肝组织和20例正常无肝硬化肝组织中CIP2A mRNA的表达,并结合肝癌临床病理因素及预后进行分析.结果 CIP2A mRNA在人肝癌细胞系Hep-G2、MHCC-97H、SMMC-7721、BEL-7402中均表达,而在永生化肝细胞系L-O2中无表达.CIP2A mRNA在肝癌组织中的阳性率(78.3%)明显高于癌旁肝组织(28.3%)和正常无肝硬化肝组织(5.0%)(P＜0.01).肝癌组织CIP2A mRNA的表达与肿瘤大小、病理分级和TNM分期相关(P＜0.05).肝癌组织中CIP2A mRNA阳性组和阴性组5年总生存率分别为44.5%和65.1%,5年无瘤生存率分别为30.1%和53.7%,差异均有统计学意义(P＜0.05).结论 CIP2A mRNA在肝癌细胞系及肝癌组织中高表达,可能在肝癌发生发展中发挥重要作用,可以作为预后预测的一个重要参考指标.
목적 검측CIP2A mRNA재간세포암중적표체,탐토기여림상병리인소화예후적관계.방법 용반정량RT-PCR검측4주인간암세포계、1주영생화간세포계이급120례간세포암조직、암방간조직화20례정상무간경화간조직중CIP2A mRNA적표체,병결합간암림상병리인소급예후진행분석.결과 CIP2A mRNA재인간암세포계Hep-G2、MHCC-97H、SMMC-7721、BEL-7402중균표체,이재영생화간세포계L-O2중무표체.CIP2A mRNA재간암조직중적양성솔(78.3%)명현고우암방간조직(28.3%)화정상무간경화간조직(5.0%)(P＜0.01).간암조직CIP2A mRNA적표체여종류대소、병리분급화TNM분기상관(P＜0.05).간암조직중CIP2A mRNA양성조화음성조5년총생존솔분별위44.5%화65.1%,5년무류생존솔분별위30.1%화53.7%,차이균유통계학의의(P＜0.05).결론 CIP2A mRNA재간암세포계급간암조직중고표체,가능재간암발생발전중발휘중요작용,가이작위예후예측적일개중요삼고지표.
Objective This study is to explore the expression of CIP2A mRNA in hepatocellular carcinoma (HCC), and its correlations with clinicopathologic features and prognosis of HCC patients.Methods CIP2A mRNA expression was analyzed in four liver cancer cell lines (Hep-G2, MHCC97,SMMC-7721 and BEI-7402), one immortalized liver cell line L-O2, neoplastic tissues and adjacent matched non-neoplastic liver tissues in 120 HCC patients and normal liver tissues of 20 cases using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). The correlations between CIP2A mRNA and clinicopathologic features and prognosis of HCC were analyzed. Results CIP2A mRNA was detected in Hep-G2, MHCC-97H, SMMC-7721 and BEL-7402, but not in L-O2.The positive rate of CIP2A mRNA expression was significantly increased in HCC tissues (78.3%)than in adjacent matched non-neoplastic liver tissues (28.3%) and normal liver tissues (5.0%,P＜0. 01). CIP2A mRNA expression was correlated with tumor size, differentiation and TNM stage (P＜0.05). Patients with positive expression of CIP2A mRNA had lower overall survival and diseasefree survival rates. Conclusions CIP2A mRNA, which is highly expressed in liver cancer cell lines and HCC tissues, may be involved in hepatocarcinogenesis. CIP2A mRNA may be a valuable biomarker for assessing the prognosis of HCC.