CAJ | 학술논문

目的研究ATM、ATR、Chk1和Chk2在人脑胶质瘤中的表达及其与肿瘤发生的关系. 方法采用SYBRTM Green实时定量PCR技术检测35例人原发脑胶质瘤组织和10例正常脑组织中的ATM、ATR、Chk1和Chk2的表达水平. 结果 ATR、Chk1和Chk2基因在各级脑胶质瘤中表达较正常脑组织升高,差异均有统计学意义(P<0.05).其中,ATR和Chk2基因表达在Ⅱ级、Ⅲ级、Ⅳ级胶质瘤组织之间差异均无统计学意义(P0.05),而Chk1,在Ⅳ级胶质瘤中的表达较Ⅱ级、Ⅲ级胶质瘤明显升高,差异均有统计学意义(P<0.05).ATM基因表达量在正常脑组织和各级脑胶质瘤中差异无统计学意义(P0.05). 结论 ATR、Chk1和Chk2在人脑胶质瘤中表达上调,说明这些基因可能与人脑胶质瘤的发生有关.其中,Chk1表达与肿瘤恶性程度有关.可作为判别胶质瘤病理级别的辅助指标.
목적 연구ATM、ATR、Chk1화Chk2재인뇌효질류중적표체급기여종류발생적관계. 방법 채용SYBRTM Green실시정량PCR기술검측35례인원발뇌효질류조직화10례정상뇌조직중적ATM、ATR、Chk1화Chk2적표체수평. 결과 ATR、Chk1화Chk2기인재각급뇌효질류중표체교정상뇌조직승고,차이균유통계학의의(P<0.05).기중,ATR화Chk2기인표체재Ⅱ급、Ⅲ급、Ⅳ급효질류조직지간차이균무통계학의의(P0.05),이Chk1,재Ⅳ급효질류중적표체교Ⅱ급、Ⅲ급효질류명현승고,차이균유통계학의의(P<0.05).ATM기인표체량재정상뇌조직화각급뇌효질류중차이무통계학의의(P0.05). 결론 ATR、Chk1화Chk2재인뇌효질류중표체상조,설명저사기인가능여인뇌효질류적발생유관.기중,Chk1표체여종류악성정도유관.가작위판별효질류병리급별적보조지표.
Objective To detect the expressions of DNA damage checkpoint genes including A TR, A TM, Chk1 and Chk2 in human primary gliomns and explore their relations with tumor progression. Methods SYBRTM Green real-time quantitative PCR was performed to detect the expressions of ATR, A TM, Chk1 and Chk2 genes in 35 cases of primary gliomas and 10 of normal brain tissues. Results In glioma tissues of various pathological grades, the expressions of the target genes, with the exception of A TM gene, were significantly increased as compared to those in normal brain tissues (P<0.05). Chk1 gene expression was significantly higher in grade Ⅳ than in grade Ⅱ and Ⅲ gliomas (P<0.05), but no significant differences were found in A TR or Chk2 gene expression between grade Ⅱ, Ⅲ and Ⅳ gliomas (P>0.05). Conclusion The up-regulation of ATR, Chk1 and Chk2 genes in primary glioma suggests their association with the pathogenesis of glioma. Chk1 expression may indicate the malignancy of glioma and help evaluate the pathological grade of glioma.