中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1331-1334
,共4页
结肠腺癌%恶病质%模型,动物
結腸腺癌%噁病質%模型,動物
결장선암%악병질%모형,동물
Colon carcinoma%Cachexia%Model,animal
目的 观察C26腺癌恶病质动物模型的自然发展过程.方法 于BALB/C小鼠接种结肠腺癌Colon26( C26)细胞,在接种后0、5、10、15、20、25 d,记录小鼠腓肠肌、附睾和去瘤体质量,检测生化指标、肿瘤坏死因子-α( TNF-α)和组织核因子-KB(NF-KB)及泛素蛋白酶体的E3连接酶(Arogin)-1和肌肉环状指蛋白(MURF)-1基因的表达.结果 本模型明显的营养不良和代谢紊乱发生在肿瘤接种后15d.荷瘤组F组较空白对照G组:白蛋白浓度分别为(14.44±1.12)、(19.80±2.45) g/L,(P＜0.01)；其他生化指标:TNF-α和NF-KB及泛素蛋白酶体的激活也有不同程度改善(P＜0.05).结论 癌症恶病质从起始发展到出现明显症状是一个较长的过程；血清白蛋白可以作为判断肿瘤患者是否发生恶病质的指标之一.
目的 觀察C26腺癌噁病質動物模型的自然髮展過程.方法 于BALB/C小鼠接種結腸腺癌Colon26( C26)細胞,在接種後0、5、10、15、20、25 d,記錄小鼠腓腸肌、附睪和去瘤體質量,檢測生化指標、腫瘤壞死因子-α( TNF-α)和組織覈因子-KB(NF-KB)及汎素蛋白酶體的E3連接酶(Arogin)-1和肌肉環狀指蛋白(MURF)-1基因的錶達.結果 本模型明顯的營養不良和代謝紊亂髮生在腫瘤接種後15d.荷瘤組F組較空白對照G組:白蛋白濃度分彆為(14.44±1.12)、(19.80±2.45) g/L,(P＜0.01)；其他生化指標:TNF-α和NF-KB及汎素蛋白酶體的激活也有不同程度改善(P＜0.05).結論 癌癥噁病質從起始髮展到齣現明顯癥狀是一箇較長的過程；血清白蛋白可以作為判斷腫瘤患者是否髮生噁病質的指標之一.
목적 관찰C26선암악병질동물모형적자연발전과정.방법 우BALB/C소서접충결장선암Colon26( C26)세포,재접충후0、5、10、15、20、25 d,기록소서비장기、부고화거류체질량,검측생화지표、종류배사인자-α( TNF-α)화조직핵인자-KB(NF-KB)급범소단백매체적E3련접매(Arogin)-1화기육배상지단백(MURF)-1기인적표체.결과 본모형명현적영양불량화대사문란발생재종류접충후15d.하류조F조교공백대조G조:백단백농도분별위(14.44±1.12)、(19.80±2.45) g/L,(P＜0.01)；기타생화지표:TNF-α화NF-KB급범소단백매체적격활야유불동정도개선(P＜0.05).결론 암증악병질종기시발전도출현명현증상시일개교장적과정；혈청백단백가이작위판단종류환자시부발생악병질적지표지일.
Objective To observe the natural developing process of the cancer cachexia-inducing C26 colon carcinoma in mice.Methods The murine colon 26 adenocarcinoma cells was inoculated subcutaneously into BAL B/C mice.On the day O,5,10,15,20,and 25 after inoculation,body weight and gastroenemius muscle with epididymal adipose were documented. Biochemical parameters,serum tumor necrosis factor-α (TNF-α),the expression of nuclear factor-KB (NF-kB) in tumor and expression of ubiquitin proteasome E3 ligase ( Atrogin-1 ) and muscle ring finger protein 1 ( MURF-1 ) genese were valuated.Results Remarkable malnutrition and metabolic disorder occured about 15 days after the tumor inoculation in this model.There was significant difference in the concentrations of serum albumin between tumor-bearing group and healthy control group at the 15th day [(14.44 ±1.12) vs ( 19.80 ±2.45) g/L,P ＜0.01].The other biochemical indicators,TNF-α,NF-kB,and Atrogin-1 and MuRF-1 had beneficial changes to varying degrees ( P＜0.05 ).Conclusion Cancer cachexia development from the inoculated tumour cells to the obvious symptoms is a long process.The serum albumin may serve as an indicator to judge cancer cachexia development.
