CAJ | 학술논문

目的比较骨髓源基质干细胞(BMSCs)和脂肪源间充质干细胞(ADSCs)向神经元样细胞诱导分化的潜力. 方法体外分离培养来自成年SD大鼠的BMSCs和ADSCs,传至第5代时加入表皮生长因子(EGF,10ng/mL)和碱性成纤维细胞生长因子(bFGF,20ng/mL),诱导6 h、12 h、24h、72 h、1周、2周后,用免疫荧光染色和Westernblot检测两种细胞的神经细胞特异性标志(巢蛋白和β.微管蛋白Ⅲ)的表达,并做统计学分析. 结果 BMSCs和ADSCs经诱导后,细胞形态发生变化,均表达神经细胞特异性标志.免疫荧光染色结果显示诱导后各时间点BMSCs和ADSCsnstin阳性率或β.tubulin Ⅲ阳性率比较差异均有统计学意义(P<0.05),且诱导后ADSCs表达nestin阳性细胞或β.tubulinⅢ的能力在各个时间点都比诱导后BMSCs强.说明诱导后的ADSCs向神经元样细胞方向分化的能力更强.Western blot结果也与免疫荧光染色结果类似. 结论两种不同来源的干细胞在分化成神经元样细胞方面的能力不同.ADSCs比BMSCs具有相对更强的神经元样细胞分化能力.
목적 비교골수원기질간세포(BMSCs)화지방원간충질간세포(ADSCs)향신경원양세포유도분화적잠력. 방법 체외분리배양래자성년SD대서적BMSCs화ADSCs,전지제5대시가입표피생장인자(EGF,10ng/mL)화감성성섬유세포생장인자(bFGF,20ng/mL),유도6 h、12 h、24h、72 h、1주、2주후,용면역형광염색화Westernblot검측량충세포적신경세포특이성표지(소단백화β.미관단백Ⅲ)적표체,병주통계학분석. 결과 BMSCs화ADSCs경유도후,세포형태발생변화,균표체신경세포특이성표지.면역형광염색결과현시유도후각시간점BMSCs화ADSCsnstin양성솔혹β.tubulin Ⅲ양성솔비교차이균유통계학의의(P<0.05),차유도후ADSCs표체nestin양성세포혹β.tubulinⅢ적능력재각개시간점도비유도후BMSCs강.설명유도후적ADSCs향신경원양세포방향분화적능력경강.Western blot결과야여면역형광염색결과유사. 결론 량충불동래원적간세포재분화성신경원양세포방면적능력불동.ADSCs비BMSCs구유상대경강적신경원양세포분화능력.
Objective To compare the differentiation potentials of bone marrow-derived stromal cells (BMSCs) and adipose-derived stromal cells (ADSCs) into neuron-like cells in vitro. Methods The fifth-passage of cultured adult SD rat BMSCs and ADSCs were induced with 10 ng/mL epidermal growth factor (EGF) and 20 ng/mL basic fibroblast growth factor (bFGF). After induction for 6, 12, 24, 72 h and 1 and 2 weeks, the cells were harvested to examine the expressions of the neural markers nestin and β-tubulin Ⅲ using immunohistoChEnistry and Western blot. Results Both the BMSCs and ADSCs underwent morphological changes into neuron-like cells and expressed the neuron-specific markers after the induction. The two cells exhibited significantly different positivity rates for nestin and β-tubulin Ⅲ after the induction (P<0.05). The ADSCs exhibited stronger ability than BMSCs to differentiate into neuron-like cells shown by greater nestin and β-tubulin Ⅲ positivity rates after the induction. Conclusion ADSCs possess stronger capacity of induced differentiation into neuron-like ceils than BMSCs in in vitro culture.