CAJ | 학술논문

目的探讨2-甲氧基雌二醇(2ME)对体外培养的U251胶质瘤细胞增殖及凋亡的影响.方法将不同浓度的2ME作用于U251不同时间,四甲基偶氮唑蓝(MTY)法检测2ME对U251细胞增殖的抑制作用;流式细胞术检测细胞凋亡和细胞周期.结果 2ME可抑制U251细胞的活性并诱导其凋亡,且在一定范围内呈剂量和时间依赖性,作用组与对照组之间的差异有统计学意义(P<0.05).细胞周期检测发现作用组G0/G1及S期细胞减少,G2/M期细胞增多,与对照组比较差异有统计学意义(P<0.05).结论 2ME可抑制U251胶质瘤细胞的增殖并诱导其凋亡.
목적 탐토2-갑양기자이순(2ME)대체외배양적U251효질류세포증식급조망적영향.방법 장불동농도적2ME작용우U251불동시간,사갑기우담서람(MTY)법검측2ME대U251세포증식적억제작용;류식세포술검측세포조망화세포주기.결과 2ME가억제U251세포적활성병유도기조망,차재일정범위내정제량화시간의뢰성,작용조여대조조지간적차이유통계학의의(P<0.05).세포주기검측발현작용조G0/G1급S기세포감소,G2/M기세포증다,여대조조비교차이유통계학의의(P<0.05).결론 2ME가억제U251효질류세포적증식병유도기조망.
Objective To explore the effects of 2 - methoxyestradiol (2 - ME) on proliferation and apoptosis of U251 glioblastoma cells in vitro. Methods U251 cells were treated with 2 - ME of dif-ferent concentrations and time in vitro, cell proliferation inhibition was detected by MTr colorimetric as-say; cell cycle arrest and apoptosis rate were measured by flow cytometry (FCM). Results Exposure to 2- ME had a strong antiproliferative effect on human glioblastoma cells and caused an increase in the population of apoptotic cells, detected by flow cytometry in U251 cell lines. A significant number of cells were blocked in the G2/M phases of the cell cycle. Concurrently, the population of cells in the Go/G<1>,and S phases decreased in U251 cell lines. Conclusions The present study showed that in vitro 2 - Meinhibits the growth of human glioblastoma cells and induces apoptosis.