CAJ | 학술논문

应用电镜技术对蕨类植物分株紫萁(Osmunda cinnamomea L. var.asiatica Fernald)精子发育过程中的生毛体和多层结构的超微结构进行了研究.生毛体在幼精子细胞中出现,正在分化的生毛体略呈球状,球状体的中央由一团染色深的颗粒状物质构成,外围分化出若干柱状体.已分化的生毛体由柱状体分散或辐射状排列构成,呈球状,球体中心不含染色深的物质.多层结构位于精子细胞内的基体和巨大线粒体之间,刚形成时仅由片层构成,片层相互平行排列形成片层带.多层结构在分化中期由微管带、片层带和蚀斑三层构成.多层结构在分化末期又形成附属微管带、嗜锇冠和嗜锇层.微管带从多层结构长出,沿细胞核的表面伸展,并与核膜之间形成复合结构.基体由柱状体转变而成,它向两端生长,在远端产生鞭毛的轴丝,在近轴端形成楔状结构.本文首次详细阐明了原始薄囊蕨分株紫萁生毛体和多层结构发育的超微结构特点,并与其他蕨类进行了比较,发现其片层带出现在微管带形成之前.
응용전경기술대궐류식물분주자기(Osmunda cinnamomea L. var.asiatica Fernald)정자발육과정중적생모체화다층결구적초미결구진행료연구.생모체재유정자세포중출현,정재분화적생모체략정구상,구상체적중앙유일단염색심적과립상물질구성,외위분화출약간주상체.이분화적생모체유주상체분산혹복사상배렬구성,정구상,구체중심불함염색심적물질.다층결구위우정자세포내적기체화거대선립체지간,강형성시부유편층구성,편층상호평행배렬형성편층대.다층결구재분화중기유미관대、편층대화식반삼층구성.다층결구재분화말기우형성부속미관대、기철관화기철층.미관대종다층결구장출,연세포핵적표면신전,병여핵막지간형성복합결구.기체유주상체전변이성,타향량단생장,재원단산생편모적축사,재근축단형성설상결구.본문수차상세천명료원시박낭궐분주자기생모체화다층결구발육적초미결구특점,병여기타궐류진행료비교,발현기편층대출현재미관대형성지전.
The ultrastructure of the blepharoplast and the multilayered structure (MLS) in the fernOsmunda cinnamomea var. asiatica Fernald have been studied by electron microscopy with respect tospermatogenesis. The blepharoplast appears in the young spermatid. The differentiating blepharoplast isapproximately a spherical body, which is composed of densely stained granular material in the center andsome cylinders outside of it. The differentiated blepharoplast is also a sphere, but without the denselystained material in the center, consisting of scattered or radially arranged cylinders. The MLS seen in thespermatid lies between the basal bodies and the giant mitochondrion. In the early developmental stage, theMLS only consists of lamellar layers, each of which runs parallel to one another and forms a strip. In themid stage, the MLS is composed of the microtubular ribbon (MTr), the lamellar layers and a layer of plaque.In the late stage, the MLS forms accessory band, osmiophilic crest and a layer of osmiophilic material.The MTr grows out from the MLS and extends along the surface of the nucleus to unite with the nuclearenvelope in a complex. The basal body coming from the cylinder produces the axoneme of the flagella inthe distal end and the wedge-shaped structure in the proximal end, respectively, In the present study, theultrastructural features of blepharoplast and the MLS of the protoleptosporangiopsida fern, O. cinnamomeavar. asiatica, have been described and compared with those of other kinds of pteridophytes in detail. Thelamellar layers appearing before the formation of the MTr was found and reported for the first time.