CAJ | 학술논문

用含中性蛋白酶8000U/g的酶制剂,制作酶制剂使用量分别为0、0.5‰、1‰、2‰和3‰的5种实验饲料.喂养平均初始重为(3.03±0.04)g的5组三重复的青鱼鱼种8周.实验在容积为80L的15只循环式水族箱中进行.实验水温为(25.0±0.5)℃.结果表明:实验饲料中酶制剂含量为0.5‰时,实验鱼的鱼体增重与摄食不含酶制剂饲料的实验组无显著差异(p＞0.05),而当饲料中酶制剂含量提高至1‰时,鱼体增重出现显著提高(p＜0.05).但饲料中酶制剂含量从1‰进一步提高至2‰和3‰,则鱼体增重不再有显著变化(p＞0.05);饲料中酶制剂含量为1‰、2‰和3‰实验组的摄食量显著高于饲料中酶制剂含量为0和0.5‰的实验组(p＜0.05).而饲料中酶制剂含量为1‰、2‰和3‰实验组的饲料系数显著低于饲料中酶制剂含量为0和0.5‰的实验组(p＜0.05);青鱼饲料中使用酶制剂对饲料干物质的表观消化率、实验鱼的肠蛋白酶活性、肠和肝胰脏淀粉酶活性、全鱼营养组成、脏体指数、肝体指数无显著影响(P＞0.05);饲料中0.5‰-3‰酶制剂含量时,实验鱼对饲料蛋白质的表观消化率均显著高于饲料中不含酶制剂的实验组(p＜0.05).青鱼鱼种肝胰脏蛋白酶活性随着饲料中酶制剂使用量从0增加0.5‰和1‰而呈逐渐升高的趋势,但饲料中酶制剂使用量继续增加则不再有显著变化.因此,在本实验条件下,饲料中使用1‰-3‰的中性蛋白酶制剂促进青鱼鱼种的生长和降低饲料系数.
용함중성단백매8000U/g적매제제,제작매제제사용량분별위0、0.5‰、1‰、2‰화3‰적5충실험사료.위양평균초시중위(3.03±0.04)g적5조삼중복적청어어충8주.실험재용적위80L적15지순배식수족상중진행.실험수온위(25.0±0.5)℃.결과표명:실험사료중매제제함량위0.5‰시,실험어적어체증중여섭식불함매제제사료적실험조무현저차이(p＞0.05),이당사료중매제제함량제고지1‰시,어체증중출현현저제고(p＜0.05).단사료중매제제함량종1‰진일보제고지2‰화3‰,칙어체증중불재유현저변화(p＞0.05);사료중매제제함량위1‰、2‰화3‰실험조적섭식량현저고우사료중매제제함량위0화0.5‰적실험조(p＜0.05).이사료중매제제함량위1‰、2‰화3‰실험조적사료계수현저저우사료중매제제함량위0화0.5‰적실험조(p＜0.05);청어사료중사용매제제대사료간물질적표관소화솔、실험어적장단백매활성、장화간이장정분매활성、전어영양조성、장체지수、간체지수무현저영향(P＞0.05);사료중0.5‰-3‰매제제함량시,실험어대사료단백질적표관소화솔균현저고우사료중불함매제제적실험조(p＜0.05).청어어충간이장단백매활성수착사료중매제제사용량종0증가0.5‰화1‰이정축점승고적추세,단사료중매제제사용량계속증가칙불재유현저변화.인차,재본실험조건하,사료중사용1‰-3‰적중성단백매제제촉진청어어충적생장화강저사료계수.
A neutral protease product with an activity of 8000 u/g was used in the experiment. A basal diet without exogenous enzyme inclusion and four test diets adding 0. 5%0, 1%0, 2%0 and 3%0 of the enzyme product respectively were prepared to feed five triplicate groups of fish with an initial average body weight of (3.03 ± 0. 04) g. The feeding trial was conducted in 15 re circulating tank systems (80L each) at a controlled water temperature of 25 ± 0. 5℃ and lasted for eight weeks. At the end of eight weeks, the weight gain of fish fed a diet with 0. 5%0 of enzyme product showed no significant difference with that of fish fed basal diet (p ＞0. 05). When fish fed a diet with 1%0 of enzyme product, the weight gain increased significantly (p ＜ 0. 05) comparing with those of fish fed basal diet and the diet with 0. 5%0 of enzyme product, but higher levels of enzyme product inclusion seemed not to improve weight gain further. Fish fed diet with 1‰, 2‰ and 3‰ of enzyme product had higher feed ingest and lower feed conversion ratio than those of fish fed basal diet or the diet with 0. 5‰ of enzyme product (p ＜0. 05). There was no effect of adding the enzyme product in the diets on the apparent digestibility of dry matter, intestine protease activity, intestine and hepato-pancreas amylase activity, body composition, Viscera index, and Hepato-pancreas index (p ＞ 0. 05). However, the apparent digestibility of dietary protein of fish fed four test diets were significantly higher than that of fish fed the basal diet (p ＜ 0. 05). The hepato-pancreas protease activity increased with increasing dietary supplementing the enzyme product up to 1‰, but above this level, it appeared to keep stable. In conclusion, under the condition of this experiment, adding 1‰ -3‰ neutral protease product to fingerling black carp diet could promote feed ingest and digest capacity of dietary protein, and as a result, improve fish growth and feed conversion.