CAJ | 학술논문

采用匀浆后丙酮抽提法从苹果梨中提取多酚氧化酶.谷胱甘肽为抑制苹果梨中多酚氧化酶的活力的最佳抑制剂.在其浓度为300μmol/L时,多酚氧化酶(PPO)的活力为50%;在其浓度为500μmol/L时,多酚氧化酶(PPO)的活力为零.谷胱甘肽、亚硫酸钠和L-半胱氨酸3种混合抑制剂对苹果梨中多酚氧化酶(PPO)的影响实验结果表明,谷胱甘肽对苹果梨中多酚氧化酶活性影响最大.
채용균장후병동추제법종평과리중제취다분양화매.곡광감태위억제평과리중다분양화매적활력적최가억제제.재기농도위300μmol/L시,다분양화매(PPO)적활력위50%;재기농도위500μmol/L시,다분양화매(PPO)적활력위령.곡광감태、아류산납화L-반광안산3충혼합억제제대평과리중다분양화매(PPO)적영향실험결과표명,곡광감태대평과리중다분양화매활성영향최대.
Crude polyphenol oxidase (PPO) in Pingguoli pear fruits was prepared through sequential steps of homogenization followed by extraction with acetone.The single inhibitory effects of glutathione,cysteine and sodium sulfite against the enzyme were evaluated according to residue enzyme activity.Subsequently,to obtain the optimal inhibitory effect,the above inhibitors were compounded and the optimal formulation was investigated using orthogonal array design.Results showed that glutathione exhibited a good inhibitory effect against this enzyme with half-inhibitory concentration of 300μmol/L and maximal inhibitory concentration of 500 μmol/L,which was stronger than that of other two inhibitors.