CAJ | 학술논문

建立高效液相色谱法同时测定丹参植株中不同部位丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B五种水溶性成分的含量.采用DiamonsilTM C18色谱柱(250mm×4.6mm,5μm),以5％冰醋酸和甲醇为流动相进行梯度洗脱,柱温:35℃,流速为1.0L/min,检测波长为288nm.丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别为0.35-21 μg·mL-1 (r=0.9999),0.3625-21.75μg·mL-1 (r=0.9997),0.32-19.2μg·mL-1 (r=0.9999),1.92-115.2μg·mL-1 (r=0.9998),8.1-483.6μg· mL-1(r=0.9998).平均回收率丹参素为99.3％(RSD 1.5％),原儿茶酸为99.7％ (RSD 1.1％),原儿茶醛为99.8％(RSD1.7％),迷迭香酸为100.1％(RSD 0.4％),丹酚酸B为99.7％ (RSD 1.5％).本方法快速简便,5组分分离良好,检测结果为综合开发利用丹参地上部分资源提供科学依据.
건립고효액상색보법동시측정단삼식주중불동부위단삼소、원인다산、원인다철、미질향산화단분산B오충수용성성분적함량.채용DiamonsilTM C18색보주(250mm×4.6mm,5μm),이5％빙작산화갑순위류동상진행제도세탈,주온:35℃,류속위1.0L/min,검측파장위288nm.단삼소、원인다산、원인다철、미질향산화단분산B적선성범위분별위0.35-21 μg·mL-1 (r=0.9999),0.3625-21.75μg·mL-1 (r=0.9997),0.32-19.2μg·mL-1 (r=0.9999),1.92-115.2μg·mL-1 (r=0.9998),8.1-483.6μg· mL-1(r=0.9998).평균회수솔단삼소위99.3％(RSD 1.5％),원인다산위99.7％ (RSD 1.1％),원인다철위99.8％(RSD1.7％),미질향산위100.1％(RSD 0.4％),단분산B위99.7％ (RSD 1.5％).본방법쾌속간편,5조분분리량호,검측결과위종합개발이용단삼지상부분자원제공과학의거.