CAJ | 학술논문

目的利用比较蛋白质组学的方法对胰腺癌患者血清和对照组血清例进行分析,寻找胰腺癌潜在的特异性候选标志物.方法采用双向差异凝胶电泳分离40例胰腺癌患者、10例慢性胰腺炎患者、10例良性肿瘤患者和40例健康体检者外周血清蛋白质,利用基质辅助激光解析电离飞行时间质谱技术对差异显著的蛋白进行鉴定.结果成功鉴定出2个胰腺癌差异表达蛋白:转甲状腺素蛋白和载脂蛋白E.该组蛋白在胰腺癌组中呈高表达,在正常对照组、慢性胰腺炎组和良性肿瘤组中呈低表达.结论双向差异凝胶电泳联合基质辅助激光解析电离飞行时间质谱技术筛选胰腺癌患者外周血清中的特异性生物标志物的方法具有较好的重复性和稳定性.检测鉴定的差异蛋白质载脂蛋白E、转甲状腺素蛋白可能是胰腺癌早期诊断潜在的血清特异性生物标志物.
목적 이용비교단백질조학적방법대이선암환자혈청화대조조혈청례진행분석,심조이선암잠재적특이성후선표지물.방법 채용쌍향차이응효전영분리40례이선암환자、10례만성이선염환자、10례량성종류환자화40례건강체검자외주혈청단백질,이용기질보조격광해석전리비행시간질보기술대차이현저적단백진행감정.결과 성공감정출2개이선암차이표체단백:전갑상선소단백화재지단백E.해조단백재이선암조중정고표체,재정상대조조、만성이선염조화량성종류조중정저표체.결론 쌍향차이응효전영연합기질보조격광해석전리비행시간질보기술사선이선암환자외주혈청중적특이성생물표지물적방법구유교호적중복성화은정성.검측감정적차이단백질재지단백E、전갑상선소단백가능시이선암조기진단잠재적혈청특이성생물표지물.
Objective A comparative proteomic method was used to analyse serum proteins between pancreatic cancer patients and control group,and to find a new protential specific marker.Methods Comparative analysis on the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients,10 chronic pancreatitis patients,10 benign tumor patients and 40 cancer-free controls was carried out by 2D differential gel electrophoresis,and differentially expressed proteins were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry.Results Two differentially expressed proteins:transthyretin and apolipoprotein E were identified.Those proteins were highly expressed in pancreatic carcinoma group compared with normal control group,chronic pancreatitis group and benign tumor group.Conclusion 2D differential gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry technology in screening specific serum biomarkers of pancreatic cancer has a well repeatability and stability.The identified protein transthyretin in this study may be as specific serum biomarkers of pancreatic carcinoma.