中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2011年
10期
743-746
,共4页
肝炎抗原,乙型%肝炎病毒,乙型%肝炎,乙型,慢性%肝硬化%肝细胞癌
肝炎抗原,乙型%肝炎病毒,乙型%肝炎,乙型,慢性%肝硬化%肝細胞癌
간염항원,을형%간염병독,을형%간염,을형,만성%간경화%간세포암
Hepatitis B antigens%Hepatitis B virus%Hepatitis B,chronic%Liver cirrhosis%Hepatoeellular carcinoma
目的 了解HBsAg定量水平在慢性乙型肝炎、乙型肝炎肝硬化、HBsAg阳性的原发性肝癌患者中的变化及其在3组患者中与HBV DNA的相关性. 方法 采集47例慢性乙型肝炎患者(乙型肝炎组),72例乙型肝炎肝硬化患者(肝硬化组)及54例肝癌患者(肝癌组)的血清标本,用雅培化学发光法进行HBsAg定量测定,荧光PCR定量法检测HBV DNA量水平.多组分析采用Kruskal-Wallis检验,两组间比较采用Mann-WhitneyU检验,相关性分析采用Spearman检验.结果 HBsAg定量值在乙型肝炎、肝硬化、肝癌组患者中的中位数分别为2361.10、1001.64、594.35IU/ml,3组间呈逐渐下降趋势,x2= 24.394,P＜0.05,差异有统计学意义;乙型肝炎组与肝硬化组比较,Z= -3.754,P＜0.05,差异有统计学意义;乙型肝炎组与肝癌组比较,Z=-4.630,P＜0.05,差异有统计学意义;而肝硬化组与肝癌组比较,差异无统计学意义.HBeAg阳性患者,HBsAg定量值在乙型肝炎组、肝硬化组、肝癌组患者的中位数分别为3259.83、1077.30、789.72 IU/ml,3组间呈下降趋势,x2= 15.643,P＜0.01,差异有统计学意义.对于HBeAg阴性患者,HBsAg定量值在乙型肝炎组、肝硬化组、肝癌组患者的中位数分别为1669.00、1001.64、582.05 IU/ml,3组间呈下降趋势,x2 =6.423,P＜0.05,差异有统计学意义.HBV DNA定量值在乙型肝炎组、肝硬化组、肝癌组患者的中位数分别为5.3579、 4.2207、1.0000 log10拷贝/ml,4分位数间距分别为(4.3579 ～6.8745)、(0.0000 ～ 5.7393)、(0.0000 ～ 4.6651)log10拷贝/ml,3组HBV DNA定量值比较,x2=31.412,P＜0.05,差异有统计学意义; HBsAg与HBV DNA在乙型肝炎组(r= 0.297,P＜0.05)、肝硬化组(r= 0.346,P＜0.05)、肝癌组(r=0.452,P＜0.05)均呈正相关.结论 HBsAg定量值在慢性乙型肝炎、肝硬化、肝癌患者中逐渐降低,且与HBV DNA水平正相关.
目的 瞭解HBsAg定量水平在慢性乙型肝炎、乙型肝炎肝硬化、HBsAg暘性的原髮性肝癌患者中的變化及其在3組患者中與HBV DNA的相關性. 方法 採集47例慢性乙型肝炎患者(乙型肝炎組),72例乙型肝炎肝硬化患者(肝硬化組)及54例肝癌患者(肝癌組)的血清標本,用雅培化學髮光法進行HBsAg定量測定,熒光PCR定量法檢測HBV DNA量水平.多組分析採用Kruskal-Wallis檢驗,兩組間比較採用Mann-WhitneyU檢驗,相關性分析採用Spearman檢驗.結果 HBsAg定量值在乙型肝炎、肝硬化、肝癌組患者中的中位數分彆為2361.10、1001.64、594.35IU/ml,3組間呈逐漸下降趨勢,x2= 24.394,P＜0.05,差異有統計學意義;乙型肝炎組與肝硬化組比較,Z= -3.754,P＜0.05,差異有統計學意義;乙型肝炎組與肝癌組比較,Z=-4.630,P＜0.05,差異有統計學意義;而肝硬化組與肝癌組比較,差異無統計學意義.HBeAg暘性患者,HBsAg定量值在乙型肝炎組、肝硬化組、肝癌組患者的中位數分彆為3259.83、1077.30、789.72 IU/ml,3組間呈下降趨勢,x2= 15.643,P＜0.01,差異有統計學意義.對于HBeAg陰性患者,HBsAg定量值在乙型肝炎組、肝硬化組、肝癌組患者的中位數分彆為1669.00、1001.64、582.05 IU/ml,3組間呈下降趨勢,x2 =6.423,P＜0.05,差異有統計學意義.HBV DNA定量值在乙型肝炎組、肝硬化組、肝癌組患者的中位數分彆為5.3579、 4.2207、1.0000 log10拷貝/ml,4分位數間距分彆為(4.3579 ～6.8745)、(0.0000 ～ 5.7393)、(0.0000 ～ 4.6651)log10拷貝/ml,3組HBV DNA定量值比較,x2=31.412,P＜0.05,差異有統計學意義; HBsAg與HBV DNA在乙型肝炎組(r= 0.297,P＜0.05)、肝硬化組(r= 0.346,P＜0.05)、肝癌組(r=0.452,P＜0.05)均呈正相關.結論 HBsAg定量值在慢性乙型肝炎、肝硬化、肝癌患者中逐漸降低,且與HBV DNA水平正相關.
목적 료해HBsAg정량수평재만성을형간염、을형간염간경화、HBsAg양성적원발성간암환자중적변화급기재3조환자중여HBV DNA적상관성. 방법 채집47례만성을형간염환자(을형간염조),72례을형간염간경화환자(간경화조)급54례간암환자(간암조)적혈청표본,용아배화학발광법진행HBsAg정량측정,형광PCR정량법검측HBV DNA량수평.다조분석채용Kruskal-Wallis검험,량조간비교채용Mann-WhitneyU검험,상관성분석채용Spearman검험.결과 HBsAg정량치재을형간염、간경화、간암조환자중적중위수분별위2361.10、1001.64、594.35IU/ml,3조간정축점하강추세,x2= 24.394,P＜0.05,차이유통계학의의;을형간염조여간경화조비교,Z= -3.754,P＜0.05,차이유통계학의의;을형간염조여간암조비교,Z=-4.630,P＜0.05,차이유통계학의의;이간경화조여간암조비교,차이무통계학의의.HBeAg양성환자,HBsAg정량치재을형간염조、간경화조、간암조환자적중위수분별위3259.83、1077.30、789.72 IU/ml,3조간정하강추세,x2= 15.643,P＜0.01,차이유통계학의의.대우HBeAg음성환자,HBsAg정량치재을형간염조、간경화조、간암조환자적중위수분별위1669.00、1001.64、582.05 IU/ml,3조간정하강추세,x2 =6.423,P＜0.05,차이유통계학의의.HBV DNA정량치재을형간염조、간경화조、간암조환자적중위수분별위5.3579、 4.2207、1.0000 log10고패/ml,4분위수간거분별위(4.3579 ～6.8745)、(0.0000 ～ 5.7393)、(0.0000 ～ 4.6651)log10고패/ml,3조HBV DNA정량치비교,x2=31.412,P＜0.05,차이유통계학의의; HBsAg여HBV DNA재을형간염조(r= 0.297,P＜0.05)、간경화조(r= 0.346,P＜0.05)、간암조(r=0.452,P＜0.05)균정정상관.결론 HBsAg정량치재만성을형간염、간경화、간암환자중축점강저,차여HBV DNA수평정상관.
Objective To investigate the discrepancy of HBsAg titre and correlation ofHBV DNA levels among patients with chronic hepatitis B (CHB),HBV-related liver cirrhosis (LC) and hepatocellular carcinoma (HCC).Methods HBsAg titre and HBV DNA in serum samples were measured among 47 CHB,72 LC and 54HCC cases using Abbott chemiluminescence and fluorescence quantitative PCR,respectively.Statistical analyses among multiple groups,between two groups and about the correlation were performed using Kruskal-Wallis test,Mann-Whitney U test and Spearman test,respectively.Results The median HBsAg titre level in serum samples decreased from 2361.10 IU/ml in CHB cohort to 1001.64 IU/ml in LC cohort and 594.35 IU/ml in HCC cohort,suggesting a statistically significant difference (x2 = 24.394,P ＜ 0.05).Moreover,HBsAg titre in CHB group was significantly higher than that in LC group (Z = -3.754,P ＜ 0.05).CHB patients had significantly higher HBsAg titre than HCC cases (Z = -4.630,P ＜ 0.05).However,there was no statistically significant difference in HBsAg titre between LC and HCC group.Among HBeAg positive patients,HBsAg titre decreased from 3259.83 IU/mlin CHB group to 1077.30 IU/ml in LC group and 789.72 IU/ml in HCC group,indicating a significant difference (x2= 15.643,P ＜ 0.01).Among HBeAg negative patients,HBsAg titre declined from 1669.00 IU/ml in CHB group to 1001.64 IU/ml in LC group and 582.05 IU/ml in HCC group,suggesting of a significant difference (x2= 6.423,P ＜ 0.05).Positive correlation between HBsAg titre and HBV DNA was found in CHB (r = 0.297,P＜ 0.05),LC(r = 0.346,P ＜ 0.05) and HCC( r = 0.452,P ＜ 0.05),respectively.Conclusion HBsAg titre level in serum decreased progressively from CHB to LC and HCC group.There were positive correlations between HBsAg titre and HBV DNA level in CHB,LC and HCC.
