CAJ | 학술논문

目的探讨E型标准株和细胞培养成功的沙眼衣原体E型临床株随着传代次数的增加其热休克蛋白60(HSP60)含量的改变.方法经McCoy细胞培养法检测出的40例E型沙眼衣原体临床株,分为首次传代出现和未出现包涵体两组,通过RT-PCR方法检测1～4次传代的HSP60含量,x2检验其与对应患者疗效的关系.结果经细胞培养成功的E型沙眼衣原体临床株传代未出现包涵体时其HSP60含量较出现包涵体时高(P＜0.05).18例首次传代出现包涵体的标本1～4次传代HSP60/16SrRNA分别为0.38±0.06、0.39±0.03、0.38±0.04、0.39±0.03；12例2次传代出现包涵体的标本1～4次传代HSP60/16SrRNA分别为1.18±0.10、0.28±0.06、0.30±0.03、0.29±0.05；10例3次传代出现包涵体的标本1～4次传代HSP60/16SrRNA分别为1.20±0.04、1.20±0.04、0.28±0.04、0.28±0.05.患者疗效对1次传代是否出现包涵体有影响,20例治疗失败患者16例1次传代未出现包涵体,1个疗程治疗后转阴患者20例中14例在1次传代出现包涵体.结论 80％治疗失败患者1次传代未见包涵体,临床株传代培养未出现包涌体时沙眼衣原体可能处于持续感染状态,其CHSP60合成增加,临床采集的衣原体标本至少应进行3次盲传以避免漏检.
목적 탐토E형표준주화세포배양성공적사안의원체E형림상주수착전대차수적증가기열휴극단백60(HSP60)함량적개변.방법 경McCoy세포배양법검측출적40례E형사안의원체림상주,분위수차전대출현화미출현포함체량조,통과RT-PCR방법검측1～4차전대적HSP60함량,x2검험기여대응환자료효적관계.결과 경세포배양성공적E형사안의원체림상주전대미출현포함체시기HSP60함량교출현포함체시고(P＜0.05).18례수차전대출현포함체적표본1～4차전대HSP60/16SrRNA분별위0.38±0.06、0.39±0.03、0.38±0.04、0.39±0.03；12례2차전대출현포함체적표본1～4차전대HSP60/16SrRNA분별위1.18±0.10、0.28±0.06、0.30±0.03、0.29±0.05；10례3차전대출현포함체적표본1～4차전대HSP60/16SrRNA분별위1.20±0.04、1.20±0.04、0.28±0.04、0.28±0.05.환자료효대1차전대시부출현포함체유영향,20례치료실패환자16례1차전대미출현포함체,1개료정치료후전음환자20례중14례재1차전대출현포함체.결론 80％치료실패환자1차전대미견포함체,림상주전대배양미출현포용체시사안의원체가능처우지속감염상태,기CHSP60합성증가,림상채집적의원체표본지소응진행3차맹전이피면루검.
Objective To detect the changes of heat shock protein 60 (HSP60) during the subcultures of standard and clinical strains of Chlamydia trachomatis (Ct),and to determine if the absence of chlamydial inclusions is associated with the persistent infection of Ct.Methods A total of 40 Ct strains isolated by cell culture from patients were included in this study and classified into 2 groups according to whether inclusions appeared after initial culture.Reverse transcription PCR was conducted to quantify the levels of HSP60 in specimens containing Ct after 1-4 subcuhures.Chi-squared test was performed to analyze the relationship between the levels of HSP60 and treatment outcomes of patients.Results The levels of HSP60 in clinical specimens containing Ct serovar E were significantly higher in subcultures prior to the appearance of inclusions than in subcultures with the appearance of inclusions (P ＜ 0.05).The ratio of HSP60:16S rRNA mRNA expression after the first,second,third,fourth passage was 0.38 ± 0.06,0.39 ± 0.03,0.38 ± 0.04 and 0.39 ±0.03 respectively in 18 specimens with inclusions appearing after the initial culture,1.18 ± 0.10,0.28 ± 0.06,0.30 ± 0.03 and 0.29 ± 0.05 respectively in 12 specimens with inclusions appearing after the second culture,1.20 ± 0.04,1.20 ± 0.04,0.28 ± 0.04 and 0.28 ± 0.05 in 10 specimens with inclusions appearing after the third culture.Whether inclusions appeared after the initial culture was associated with the treatment outcome of patients.Inclusions were undetected after the initial culture in 16 of 20 specimens from patients with poor response to treatment,but observed in 14 of 20 specimens from patients who tested negative for Ct after one course of treatment.Conclusions It is implicated that no inclusions form after the initial culture in 80％ of specimens from patients experiencing treatment failure.The Ct strains whose inclusions do not form after passages may be in a persistent state,and the expression of HSP60 is high in these strains.Specimens should be subjected to at least 3 blind passages to avoid missed diagnosis of Ct infection.