中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
6期
457-462
,共6页
凌家炜%方丛%徐艳文%庄广伦%曹宝强
凌傢煒%方叢%徐豔文%莊廣倫%曹寶彊
릉가위%방총%서염문%장엄륜%조보강
基因%多态性%单核苷酸%核酸扩增技术%非整倍性%比较基因组杂交
基因%多態性%單覈苷痠%覈痠擴增技術%非整倍性%比較基因組雜交
기인%다태성%단핵감산%핵산확증기술%비정배성%비교기인조잡교
Gene%Polymorphism,single nucleotide%Nucleic acid amplification techniques%Aneuploidy%Comparative genomic hybridization
目的 评估利用微阵列-比较基因组杂交技术检测少量细胞非整倍体的准确率及相关影响因素.方法 结合10K 2.0单核苷酸多态性(SNP)基因分型芯片平台与多重置换扩增技术(MDA),计算并比较扩增模板为1~10个细胞时各染色体拷贝数分析的准确率,评估影响芯片平台的拷贝数准确率的有关因素及其对染色体拷贝数异常的实际分辨率.结果 使用MDA-DNA作参照时,拷贝数分析的准确率[(79.3±2.9)%~(100.0±1.7)%]高于使用gDNA作参照时的准确率[(66.7±3.4)%~(89.5±3.3)%](P<0.001).随着模板增加至10细胞,芯片可在1 M平滑化处理的同时获得94%的分析准确率.对于单细胞MDA产物,缺失型非整倍体具有比获得型非整倍体更高的分析准确率[1C组(71.9±4.1)%~(95.5~2.0)%;1C-sDel-4组(81.4±3.7)%~(99.6±2.8)%],各组间差异均有统计学意义(P<0.01).结论 10K 2.0 SNP基因分型芯片平台结合多重置换扩增技术可有效对少量细胞进行非整倍体检测,选择MDA-DNA作为参照是提高分析准确率的最关键因素,而增加细胞模板与提升分析中的平滑化参数(即降低芯片的分辨率要求)也有助于改善拷贝数准确率,在同样的条件下,芯片更容易准确检出缺失型非整倍体.
目的 評估利用微陣列-比較基因組雜交技術檢測少量細胞非整倍體的準確率及相關影響因素.方法 結閤10K 2.0單覈苷痠多態性(SNP)基因分型芯片平檯與多重置換擴增技術(MDA),計算併比較擴增模闆為1~10箇細胞時各染色體拷貝數分析的準確率,評估影響芯片平檯的拷貝數準確率的有關因素及其對染色體拷貝數異常的實際分辨率.結果 使用MDA-DNA作參照時,拷貝數分析的準確率[(79.3±2.9)%~(100.0±1.7)%]高于使用gDNA作參照時的準確率[(66.7±3.4)%~(89.5±3.3)%](P<0.001).隨著模闆增加至10細胞,芯片可在1 M平滑化處理的同時穫得94%的分析準確率.對于單細胞MDA產物,缺失型非整倍體具有比穫得型非整倍體更高的分析準確率[1C組(71.9±4.1)%~(95.5~2.0)%;1C-sDel-4組(81.4±3.7)%~(99.6±2.8)%],各組間差異均有統計學意義(P<0.01).結論 10K 2.0 SNP基因分型芯片平檯結閤多重置換擴增技術可有效對少量細胞進行非整倍體檢測,選擇MDA-DNA作為參照是提高分析準確率的最關鍵因素,而增加細胞模闆與提升分析中的平滑化參數(即降低芯片的分辨率要求)也有助于改善拷貝數準確率,在同樣的條件下,芯片更容易準確檢齣缺失型非整倍體.
목적 평고이용미진렬-비교기인조잡교기술검측소량세포비정배체적준학솔급상관영향인소.방법 결합10K 2.0단핵감산다태성(SNP)기인분형심편평태여다중치환확증기술(MDA),계산병비교확증모판위1~10개세포시각염색체고패수분석적준학솔,평고영향심편평태적고패수준학솔적유관인소급기대염색체고패수이상적실제분변솔.결과 사용MDA-DNA작삼조시,고패수분석적준학솔[(79.3±2.9)%~(100.0±1.7)%]고우사용gDNA작삼조시적준학솔[(66.7±3.4)%~(89.5±3.3)%](P<0.001).수착모판증가지10세포,심편가재1 M평활화처리적동시획득94%적분석준학솔.대우단세포MDA산물,결실형비정배체구유비획득형비정배체경고적분석준학솔[1C조(71.9±4.1)%~(95.5~2.0)%;1C-sDel-4조(81.4±3.7)%~(99.6±2.8)%],각조간차이균유통계학의의(P<0.01).결론 10K 2.0 SNP기인분형심편평태결합다중치환확증기술가유효대소량세포진행비정배체검측,선택MDA-DNA작위삼조시제고분석준학솔적최관건인소,이증가세포모판여제승분석중적평활화삼수(즉강저심편적분변솔요구)야유조우개선고패수준학솔,재동양적조건하,심편경용역준학검출결실형비정배체.
Objective To evaluate the accuracy and influencing factors in use of array-comparative genomic hybridization (CGH) combined with multiple displacement amplification (MDA) for aneuploidy screening in a small number of ceils.Methods 10K 2.0 SNP mapping array platform and MDA were used in combination to analyze the copy number concordance of MDA product from a small number of cell templates (1 - 10).Related factors that influenced the copy number concordance and the practical resolution of SNP array were then estimated.Results The copy number concordance was higher when MDA-DNA rather than gDNA was used as a reference [(79.3±2.9)%-(100.0±1.7)% vs (66.7±3.4)%-(89.5±3.3)%] (P<0.001).The copy number concordance along the whole genome was up to 94% when 10 cells were used as template under 1 M smoothing treatment.For MDA products from single cells, the concordance was higher for aneuploidy due to chromosome "loss" than for those due to chromosome "gain" [(71.9±4.1)%-(95.5±2.0)% in group 1C vs (81.4±3.7)%-(99.6±2.8)% in group 1C-sDel-4] (P<0.01).Conclusions 10K 2.0 SNP mapping array in combination with MDA is a reliable and highly efficient method for aneuploidy screening in a small number of cells.The use of MDA- DNA as reference, increasing the number of template cells and widening smoothing size can improve the copy number concordance.Accurate detection of a "loss" variation is more readily than as for a "gain" variation under the same condition.