糖皮质激素治疗二氧化氮致大鼠急性肺水肿实验初步研究
당피질격소치료이양화담치대서급성폐수종실험초보연구
Preliminary experimental research on glucocorticoid for treatment of nitrogen dioxide induced acute pulmonary edema in rats
  • 万方数据
    中华劳动卫生职业病杂志 중화노동위생직업병잡지
    CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
    2010年 11期 822-826 ,共5页

    张巡淼%孙道远%唐亮%阮艳君

    장순묘%손도원%당량%원염군

    糖皮质激素类%二氧化氮%肺水肿%白细胞介素类 糖皮質激素類%二氧化氮%肺水腫%白細胞介素類
    당피질격소류%이양화담%폐수종%백세포개소류
    Glucocorticoids%Nitrogen dioxide%Pulmonary edema%Interleukins
    目的 探讨糖皮质激素对二氧化碳(NO2)所致大鼠急性肺水肿的治疗效果.方法 将SD大鼠随机分为空白对照组、染毒组及大、中、小剂量糖皮质激素治疗组,每组6只.空白对照组予净空气平衡30 min,染毒组及大、中、小剂量糖皮质激素治疗组在NO2染毒后分别给予生理盐水2.5 mg/kg和地塞米松6.0、3.0、1.0 mg/kg.测定大鼠肺湿干重比(W/D),用ELISA法测定血浆心房利尿钠肽(ANP)含量、全血超氧化物歧化酶(SOD)活力及血浆中白细胞介素-6(IL-6)、IL-10、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)的含量.结果 与空白对照组比较,大、中、小剂量激素治疗组及染毒组大鼠肺W/D明显升高,差异有统计学意义(P<0.05);与染毒组比较,大、中、小剂量激素治疗组大鼠肺W/D明显降低,差异有统计学意义(P<0.05).与空白对照组比较,大、中、小剂量激素治疗组及染毒组大鼠全血中SOD活力明显降低,差异有统计学意义(P<0.05);与染毒组比较,大、中、小剂量激素治疗组全血中SOD活力无明显改变,差异无统计学意义(P>0.05).与空白对照组比较,染毒组大鼠血浆中ANP含量明显升高,差异有统计学意义(P<0.05);与染毒组比较,大、中、小剂量激素治疗组大鼠血浆中ANP含量明显降低,差异有统计学意义(P<0.05).与染毒组[(68.55±27.84)pg/ml]比较,大、中、小剂量激素治疗组大鼠血浆中TNFα含量[分别为(27.04±8.19)、(40.10±9.09)、(39.76±9.60)pg/ml]明显降低,差异有统计学意义(P<0.05).与染毒组[(29.29±9.31)pg/ml]比较,大、中剂量激素治疗组大鼠血浆中IL-6含量[分别为(15.97±6.18)、(19.69±5.52)pg/ml]降低,差异有统计学意义(P<0.05).与染毒组[(44.38±9.19)pg/ml]比较,大、中、小剂量激素治疗组大鼠血浆中IL-10含量[(23.24±5.14)、(27.78±8.17)、(33.29±10.42)pg/ml]降低,差异有统计学意义(P<0.05).与染毒组[(30.83±6.82)pg/ml]比较,大、中剂量激素治疗组大鼠血浆中IFN-γ含量[分别为(7.21±4.55)、(19.23±4.35)pg/ml]降低,差异有统计学意义(P<0.05).结论 大、中、小剂量糖皮质激素均具有改善肺泡壁和毛细血管的通透性及非特异性的抗炎作用,对肺水肿有明显的治疗作用,在降低炎性因子方面,以大、中剂量激素治疗组效果更为明显.
    목적 탐토당피질격소대이양화탄(NO2)소치대서급성폐수종적치료효과.방법 장SD대서수궤분위공백대조조、염독조급대、중、소제량당피질격소치료조,매조6지.공백대조조여정공기평형30 min,염독조급대、중、소제량당피질격소치료조재NO2염독후분별급여생리염수2.5 mg/kg화지새미송6.0、3.0、1.0 mg/kg.측정대서폐습간중비(W/D),용ELISA법측정혈장심방이뇨납태(ANP)함량、전혈초양화물기화매(SOD)활력급혈장중백세포개소-6(IL-6)、IL-10、종류배사인자-α(TNF-α)、γ-간우소(IFN-γ)적함량.결과 여공백대조조비교,대、중、소제량격소치료조급염독조대서폐W/D명현승고,차이유통계학의의(P<0.05);여염독조비교,대、중、소제량격소치료조대서폐W/D명현강저,차이유통계학의의(P<0.05).여공백대조조비교,대、중、소제량격소치료조급염독조대서전혈중SOD활력명현강저,차이유통계학의의(P<0.05);여염독조비교,대、중、소제량격소치료조전혈중SOD활력무명현개변,차이무통계학의의(P>0.05).여공백대조조비교,염독조대서혈장중ANP함량명현승고,차이유통계학의의(P<0.05);여염독조비교,대、중、소제량격소치료조대서혈장중ANP함량명현강저,차이유통계학의의(P<0.05).여염독조[(68.55±27.84)pg/ml]비교,대、중、소제량격소치료조대서혈장중TNFα함량[분별위(27.04±8.19)、(40.10±9.09)、(39.76±9.60)pg/ml]명현강저,차이유통계학의의(P<0.05).여염독조[(29.29±9.31)pg/ml]비교,대、중제량격소치료조대서혈장중IL-6함량[분별위(15.97±6.18)、(19.69±5.52)pg/ml]강저,차이유통계학의의(P<0.05).여염독조[(44.38±9.19)pg/ml]비교,대、중、소제량격소치료조대서혈장중IL-10함량[(23.24±5.14)、(27.78±8.17)、(33.29±10.42)pg/ml]강저,차이유통계학의의(P<0.05).여염독조[(30.83±6.82)pg/ml]비교,대、중제량격소치료조대서혈장중IFN-γ함량[분별위(7.21±4.55)、(19.23±4.35)pg/ml]강저,차이유통계학의의(P<0.05).결론 대、중、소제량당피질격소균구유개선폐포벽화모세혈관적통투성급비특이성적항염작용,대폐수종유명현적치료작용,재강저염성인자방면,이대、중제량격소치료조효과경위명현.
    Objective To investigate the therapeutic effect of glucocorticoids on the acute pulmonary edema in rats induced by nitrogen dioxide(NO2). Methods Thirty SD female rats were randomly equally divided into 5 groups: normal control group, NO2 exposed group, high-,middle-and low-dose of glucocorticoids treated group (6 rats per group) . 6 rats in the normal control group were exposed to room air for 30 min, and the other rats to NO2. 18 rats in the glucocorticoids group were treated with different doses of dexamethasone (6.0, 3.0, 1.0 mg/kg), while the rats in the NO2 poisoning group were treated with normal saline (2.5 mg/kg).The lung wet/dry (W/D) weight ratio was calculated, and plasma atrial natriuretic peptide (ANP) levels, superoxide dismutase (SOD) activity from whole blood, plasma interleukin 6 (IL-6), interleukin 10 ( IL- 10), tumor necrosis factor- α (TNF-α) and Interferon-γ (IFN-γ)were detected by enzyme-linked immunosorbent assay (ELISA). Results The lung W/D ratios were increased significantly in glucocorticoids treated group and NO2-exposed group compared with normal control group (P<0.05), while they were significantly reduced in glucocorticoids treated group as compared with NO2-exposed group (P<0.05). SOD activity in whole blood in glucocorticoids treated group and NO2-exposed group was significantly lower than that of normal control group (P<0.05), while it was no significant difference between that of glucocorticoids treated group and NO2 -exposed group (P>0.05). Plasma ANP was significantly increased in NO2-exposed group compared with normal control group (P<0.05), while it was significantly decreased in glucocorticoids treated group compared with NO2 -exposed group(P>0.05 ). Plasma TNF-α of high-,middle- and low- dose of glucocorticoids treated group[(27.04±8.19), (40.10±9.09), (39.76±9.60) pg/ml] was decreased significantly as compared with NO2-exposed group (68.55 ±27.84 pg/ml) (P<0.05). Plasma IL-6 in high-and middle-dose of glucocorticoids treated group [( 15.97±6.18 )、 ( 19.69±5.52 ) pg/ml] was significantly decreased as compared to NO2-exposed group[(29.29±9.31 ) pg/ml] (P<0.05). Plasma IL- 10 in high-,middle-and low- dose of glucocorticoids treated group[(23.24±5.14), (27.78 ±8.17), (33.29±10.42) pg/ml] was significantly reduced compared with NO2-exposed group [(44.38 ±9.19) pg/ml] (P<0.05). Plasma IFN-γ in high-and middle-dose of glucocorticoids treated group [(7.21±4.55 ), ( 19.23±4.35 ) pg/ml] was reduced compared with NO2-exposed group[(30.83±6.82 ) pg/ml](P<0.05 ). Conclusion High-,middle-,low -dose glucocorticoids all can improve the permeability of alveolar wall and capillary, and have nonspecific anti-inflammatory effects. The therapeutic effects on pulmonary edema re significant. High and middle dose of glucocorticoids treated group are more useful for decreased inflammatory factors.
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