CAJ | 학술논문

目的通过对比研究成纤维细胞生长因子4(fibroblast growth factor-4,FGF-4)基因在阿糖胞苷所致的大鼠拇指多指畸形胚胎鼠的肢芽组织中的表达情况,初步阐明其在畸形鼠体内的表达规律,探索多指畸形的形成机制.方法选取2～3个月龄SD大鼠66只,于每日下午6时按雌雄2:1合笼,次日晨8时取出,查见阴道栓者定为孕期第0.5天.将怀孕大鼠随机分成两组:给药组和对照组.在怀孕第10.5天腹腔注射阿糖胞苷(100 mg/kg)建立拇指多指畸形鼠的动物模型.选择不同怀孕时间的大鼠(10.5、11、11.5、12、12.5 d),颈椎脱臼处死,低温无菌条件剪取肢芽组织迅速冰冻.采用肢芽组织提取mRNA,RT-PCR半定量分析方法检测不同胚龄肢芽组织FGF-4 mRNA的表达变化.结果 FGF-4 mRNA的表达及其蛋白的表达在给药组胚胎鼠肢芽中比对照组增多,差异有统计学意义(P＜0.01).结论阿糖胞苷可以导致大鼠胚胎鼠拇指多指畸形.FGF-4在阿糖胞苷所致的拇指多指畸形鼠的肢芽中呈现高表达.
목적 통과대비연구성섬유세포생장인자4(fibroblast growth factor-4,FGF-4)기인재아당포감소치적대서무지다지기형배태서적지아조직중적표체정황,초보천명기재기형서체내적표체규률,탐색다지기형적형성궤제.방법 선취2～3개월령SD대서66지,우매일하오6시안자웅2:1합롱,차일신8시취출,사견음도전자정위잉기제0.5천.장부잉대서수궤분성량조:급약조화대조조.재부잉제10.5천복강주사아당포감(100 mg/kg)건립무지다지기형서적동물모형.선택불동부잉시간적대서(10.5、11、11.5、12、12.5 d),경추탈구처사,저온무균조건전취지아조직신속빙동.채용지아조직제취mRNA,RT-PCR반정량분석방법검측불동배령지아조직FGF-4 mRNA적표체변화.결과 FGF-4 mRNA적표체급기단백적표체재급약조배태서지아중비대조조증다,차이유통계학의의(P＜0.01).결론 아당포감가이도치대서배태서무지다지기형.FGF-4재아당포감소치적무지다지기형서적지아중정현고표체.
Objective To observe the expression offibroblast growth factor4 (FGF-4) in polydactyly of the thumb induced by cytarabine and investigate the pathogenesis of polydactyly. Methods Sixty-six 2 to 3 months old Sprague Dawley rats were caged together daily from 6 pm to 8 am next morning by a male:female ratioof 1:2.The day that a vaginal plug was found was considered as embryonic day 0.5 ( E0.5).30 pregnant rats were assigned to the experimental group, while another 30 were assigned as controls.At day E10.5 intraperitoneal injection of cytarabine ( 100 mg/kg) was carried out in the experimental rats to induce polydactyly and syndactyly in the pupa.Normal saline was injected to the control rats.E10.5 to E12.5 embryos were removed from the pregnant female rats and limb buds were taken out and fast frozen.mRNA was extracted from the limb buds.The expression of FGF-4 mRNA was quantified using RT-PCR. Results In the experimental group FGF-4 expression in the limb buds was significantly higher and lasted much longer than the control group (P ＜0.01).Conclusion The rat embryo's polydactyly of the thumb can be induced by cytarabine.FGF-4 expression in the limb buds in the polydactyly embryo is increased.