中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
6期
553-556
,共4页
汪枫桦%姜媛%王雯秋%胡玮婷%苏梦茹%陈训劼%顾青%孙晓东
汪楓樺%薑媛%王雯鞦%鬍瑋婷%囌夢茹%陳訓劼%顧青%孫曉東
왕풍화%강원%왕문추%호위정%소몽여%진훈할%고청%손효동
脉络膜新生血管化/治疗%受体,CXCR4/药物作用%血管内皮生长因子类%趋化因子CXCL12%疾病模型,动物
脈絡膜新生血管化/治療%受體,CXCR4/藥物作用%血管內皮生長因子類%趨化因子CXCL12%疾病模型,動物
맥락막신생혈관화/치료%수체,CXCR4/약물작용%혈관내피생장인자류%추화인자CXCL12%질병모형,동물
Choroidal neovascularization/therapy%Receptors,CXCR4/ drug effects%Vascular endothelial growth factors%Chemokine CXCL12%Disease models,animal
目的 观察玻璃体腔联合注射CXCR4抑制剂AMD3100与抗血管内皮生长因子(VEGF)抗体对实验性脉络膜新生血管(CNV)形成的干预作用.方法 选取48只棕色挪威(BN)大鼠随机分为AF564干预实验组(A组)、AMD3100干预实验组(B组)、联合干预实验组(C组)、磷酸盐缓冲液(PBS)对照组(D组),每组均为12只大鼠,左眼为实验眼.采用氪红激光光凝建立CNV模型.激光光凝后即刻玻璃体腔分别注射抗鼠VEGF抗体(AF564)、CXCR4特异性抑制剂AMD3100、抗鼠VEGF抗体及AMD3100、PBS各5μl.激光光凝后14 d行荧光素眼底血管造影(FFA),病理组织切片及脉络膜血管铺片检查.观察不同组别大鼠荧光渗漏程度以及CNV相对厚度和面积的变化.结果 激光光凝后14d,A、B、C、D组荧光渗漏评分分别为2.16±0.91、2.16±0.91、1.92±1.03、1.39±0.93.A、B、C组荧光渗漏较D组荧光渗漏明显受抑制,差异均有统计学意义(F=12.91,P＜0.001)；C组荧光渗漏程度低于A、B组,差异有统计学意义(F=9.21,P＜0.05).组织病理学检查显示,激光光凝后14 d,A、B、C、D组CNV相对厚度分别为1.82±0.11、1.90±0.22、1.12±0.12、2.82±0.29.A、B、C组相对CNV厚度与D组CNV相对厚度比较,差异均有统计学意义(F=5.92,P＜0.001)；C组CNV相对厚度明显变薄,与A、B组CNV相对厚度比较,差异均有统计学意义(F=5.16,P＜0.05).脉络膜血管铺片结果显示,A、B、C、D组CNV面积分别为(8204±122)、(9332±211)、(6533±101)、(13 644±255)μm2.A、B、C组CNV面积较D组CNV面积明显减少,差异均有统计学意义(F=147.50,P＜0.001)；C组CNV面积与A、B组CMV面积比较,差异有统计学意义(F=112.60,P＜0.05).结论 CXCR4抑制剂及抗VEGF抗体联合使用,可显著抑制激光诱导的CNV形成.
目的 觀察玻璃體腔聯閤註射CXCR4抑製劑AMD3100與抗血管內皮生長因子(VEGF)抗體對實驗性脈絡膜新生血管(CNV)形成的榦預作用.方法 選取48隻棕色挪威(BN)大鼠隨機分為AF564榦預實驗組(A組)、AMD3100榦預實驗組(B組)、聯閤榦預實驗組(C組)、燐痠鹽緩遲液(PBS)對照組(D組),每組均為12隻大鼠,左眼為實驗眼.採用氪紅激光光凝建立CNV模型.激光光凝後即刻玻璃體腔分彆註射抗鼠VEGF抗體(AF564)、CXCR4特異性抑製劑AMD3100、抗鼠VEGF抗體及AMD3100、PBS各5μl.激光光凝後14 d行熒光素眼底血管造影(FFA),病理組織切片及脈絡膜血管鋪片檢查.觀察不同組彆大鼠熒光滲漏程度以及CNV相對厚度和麵積的變化.結果 激光光凝後14d,A、B、C、D組熒光滲漏評分分彆為2.16±0.91、2.16±0.91、1.92±1.03、1.39±0.93.A、B、C組熒光滲漏較D組熒光滲漏明顯受抑製,差異均有統計學意義(F=12.91,P＜0.001)；C組熒光滲漏程度低于A、B組,差異有統計學意義(F=9.21,P＜0.05).組織病理學檢查顯示,激光光凝後14 d,A、B、C、D組CNV相對厚度分彆為1.82±0.11、1.90±0.22、1.12±0.12、2.82±0.29.A、B、C組相對CNV厚度與D組CNV相對厚度比較,差異均有統計學意義(F=5.92,P＜0.001)；C組CNV相對厚度明顯變薄,與A、B組CNV相對厚度比較,差異均有統計學意義(F=5.16,P＜0.05).脈絡膜血管鋪片結果顯示,A、B、C、D組CNV麵積分彆為(8204±122)、(9332±211)、(6533±101)、(13 644±255)μm2.A、B、C組CNV麵積較D組CNV麵積明顯減少,差異均有統計學意義(F=147.50,P＜0.001)；C組CNV麵積與A、B組CMV麵積比較,差異有統計學意義(F=112.60,P＜0.05).結論 CXCR4抑製劑及抗VEGF抗體聯閤使用,可顯著抑製激光誘導的CNV形成.
목적 관찰파리체강연합주사CXCR4억제제AMD3100여항혈관내피생장인자(VEGF)항체대실험성맥락막신생혈관(CNV)형성적간예작용.방법 선취48지종색나위(BN)대서수궤분위AF564간예실험조(A조)、AMD3100간예실험조(B조)、연합간예실험조(C조)、린산염완충액(PBS)대조조(D조),매조균위12지대서,좌안위실험안.채용극홍격광광응건립CNV모형.격광광응후즉각파리체강분별주사항서VEGF항체(AF564)、CXCR4특이성억제제AMD3100、항서VEGF항체급AMD3100、PBS각5μl.격광광응후14 d행형광소안저혈관조영(FFA),병리조직절편급맥락막혈관포편검사.관찰불동조별대서형광삼루정도이급CNV상대후도화면적적변화.결과 격광광응후14d,A、B、C、D조형광삼루평분분별위2.16±0.91、2.16±0.91、1.92±1.03、1.39±0.93.A、B、C조형광삼루교D조형광삼루명현수억제,차이균유통계학의의(F=12.91,P＜0.001)；C조형광삼루정도저우A、B조,차이유통계학의의(F=9.21,P＜0.05).조직병이학검사현시,격광광응후14 d,A、B、C、D조CNV상대후도분별위1.82±0.11、1.90±0.22、1.12±0.12、2.82±0.29.A、B、C조상대CNV후도여D조CNV상대후도비교,차이균유통계학의의(F=5.92,P＜0.001)；C조CNV상대후도명현변박,여A、B조CNV상대후도비교,차이균유통계학의의(F=5.16,P＜0.05).맥락막혈관포편결과현시,A、B、C、D조CNV면적분별위(8204±122)、(9332±211)、(6533±101)、(13 644±255)μm2.A、B、C조CNV면적교D조CNV면적명현감소,차이균유통계학의의(F=147.50,P＜0.001)；C조CNV면적여A、B조CMV면적비교,차이유통계학의의(F=112.60,P＜0.05).결론 CXCR4억제제급항VEGF항체연합사용,가현저억제격광유도적CNV형성.
Objective To observe the effects of CXCR4 inhibitor (AMD3100) combined with antivascular endothelial growth factor (VEGF) antibody on experimental choroidal neovascularization.Methods Choroidal neovascularization (CNV) was induced in 48 Brown-Norway (BN) rats by Krypton red laser photocoagulation,and those rats were randomly divided into AF564 group (group A),AMD3100 group (group B),combined treatment group (group C) and PBS group (group D),12 rats in each group.Left eyes were the experimental eyes.The rats of group A - D received intravitreal injection of 5μl of AF564,AMD3100,AF564/AMD3100 and PBS after laser photocoagulation respectively.Fourteen days after photocoagulation,fundus fluorescein angiography (FFA),pathological section analysis and choroidal vascular wholemount were used to observe the degree of fluorescein leakage,the relative thickness and areas of CNV.Results Fourteen days after photocoagulation,the scores of fluorescein leakage in group A - D were 2.16±0.91,2.16±0.91,1.92±1.03,1.39±0.93 respectively.Fluorescein leakage in group A - C was obviously reduced compared to group D (F =12.91,P＜0.001 ),while fluorescein leakage in group C was reduced compared to group A and B (F=9.21,P＜0.05 ).The CNV relative thicknesses in group A-D were 1.82±0.11,1.90±0.22,1.12±0.12,2.82±0.29 respectively.Group A -C had thinner CNV compared to group D (F=5.92,P＜0.001),while group C had thinner CNV compared to group A and B (F=5.16,P＜0.05).The CNV areas in group A - D were (8204± 122),(9332±211),(6533± 101),and (13644±255) μm2 respectively.Group A - C had smaller CNV area compared to group D (F=147.50,P＜0.001),while group C had smaller CNV area compared to group A and B (F=112.60,P＜0.05).Conclusion Combined treatment with CXCR4 inhibitor and anti-VEGF antibody can inhibit laser-induced CNV significantly.
